Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions
Fecha
2017Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1371/journal.pone.0174748
Resumen
In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism
has been examined in poplar trees. The overexpression of the gene and potential
effects on growth and biomass production were compared between trees growing in a
growth chamber under controlled conditions and trees growing in a field trial during two
growth seasons. Ten-week-old transgenic pop ...
[++]
In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism
has been examined in poplar trees. The overexpression of the gene and potential
effects on growth and biomass production were compared between trees growing in a
growth chamber under controlled conditions and trees growing in a field trial during two
growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed
controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form
of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates
such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of
the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin
were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key
enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However,
the results in the field trial experiment diverged from those observed in the chamber
system. The lines overexpressing PpDof5 showed attenuated growth during the two growing
seasons and no modification of carbon or nitrogen metabolism. These results were not
associated with a decrease in the expression of the transgene, but they can be ascribed to
the nitrogen available in the field soil compared to that available for growth under controlled
conditions. This work highlights the paramount importance of testing transgenic lines in field
trials. [--]
Materias
Dof5,
Hybrid poplars,
Gene overexpression
Editor
Public Library of Science
Publicado en
Plos One, 12(4): e0174748
Departamento
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural /
Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Versión del editor
Entidades Financiadoras
This work was supported by grants from the Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R) and Junta de Andalucia (BIO-474).