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dc.creatorRueda López, Marinaes_ES
dc.creatorPascual, María Belénes_ES
dc.creatorPallero, Mercedeses_ES
dc.creatorHenao, Luisa Maríaes_ES
dc.creatorLasa Larrea, Bertaes_ES
dc.creatorJáuregui Mosquera, Ivánes_ES
dc.creatorAparicio Tejo, Pedro Maríaes_ES
dc.creatorCánovas, Francisco M.es_ES
dc.creatorÁvila, Concepciónes_ES
dc.date.accessioned2018-09-06T12:20:25Z
dc.date.available2018-09-06T12:20:25Z
dc.date.issued2017
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2454/30562
dc.description.abstractIn this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.en
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R) and Junta de Andalucia (BIO-474).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 12(4): e0174748en
dc.rights© 2017 Rueda-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDof5en
dc.subjectHybrid poplarsen
dc.subjectGene overexpressionen
dc.titleOverexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditionsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Ciencias del Medio Naturales_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1371/journal.pone.0174748
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BIO2015-69285en
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0174748
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2017 Rueda-López et al. This is an
open access article distributed under the terms of
the Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.
Except where otherwise noted, this item's license is described as © 2017 Rueda-López et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.