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    Recovery of nonpathogenic mutant bacteria from tumors caused by several Agrobacterium tumefaciens strains: a frequent event?

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    Fecha
    2009
    Autor
    Llop, Pablo 
    Murillo Martínez, Jesús Upna
    Lastra, Beatriz 
    López, María Milagros 
    Versión
    Acceso abierto / Sarbide irekia
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    Artículo / Artikulua
    Versión
    Versión publicada / Argitaratu den bertsioa
    Impacto
     
     
     
    10.1128/aem.01867-08
     
     
     
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    Resumen
    We have evaluated the interaction that bacterial genotypes and plant hosts have with the loss of pathogenicity in tumors, using seven Agrobacterium tumefaciens strains inoculated on 12 herbaceous and woody hosts. We performed a screening of the agrobacteria present inside the tumors, looking for nonpathogenic strains, and found a high variability of those strains in this niche. To verify the orig ... [++]
    We have evaluated the interaction that bacterial genotypes and plant hosts have with the loss of pathogenicity in tumors, using seven Agrobacterium tumefaciens strains inoculated on 12 herbaceous and woody hosts. We performed a screening of the agrobacteria present inside the tumors, looking for nonpathogenic strains, and found a high variability of those strains in this niche. To verify the origin of the putative nonpathogenic mutant bacteria, we applied an efficient, reproducible, and specific randomly amplified polymorphic DNA analysis method. In contrast with previous studies, we recovered a very small percentage (0.01%) of nonpathogenic strains that can be considered true mutants. Of 5,419 agrobacterial isolates examined, 662 were nonpathogenic in tomato, although only 7 (from pepper and tomato tumors induced by two A. tumefaciens strains) could be considered to derive from the inoculated strain. Six mutants were affected in the transferred DNA (T-DNA) region; one of them contained IS426 inserted into the iaaM gene, whereas the whole T-DNA region was apparently deleted in three other mutants, and the virulence of the remaining two mutants was fully restored with the T-DNA genes as well. The plasmid profile was altered in six of the mutants, with changes in the size of the Ti plasmid or other plasmids and/or the acquisition of new plasmids. Our results also suggest that the frequent occurrence of nonpathogenic clones in the tumors is probably due to the preferential growth of nonpathogenic agrobacteria, of either endophytic or environmental origin, but different from the bacterial strain inducing the tumor. [--]
    Materias
    Agrobacterium tumefaciens, Nonpathogenic mutant bacteria, Tumors
     
    Editor
    American Society for Microbiology
    Publicado en
    Applied and Environmental Microbiology, Oct. 2009, Vol. 75, No. 20, p. 6504–6514
    Departamento
    Universidad Pública de Navarra. Departamento de Producción Agraria / Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
     
    Versión del editor
    https://doi.org/10.1128/aem.01867-08
    URI
    https://hdl.handle.net/2454/31937
    Entidades Financiadoras
    This research was supported partially by project INIA (grant SC93- 117) of the Spanish Ministry of Agriculture and by COST Action 873 (European Union). Pablo Llop has a contract from the Ministry of Education and INIA, with funding from the European Union (Social European Funding).
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