• Login
    View Item 
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • Dpto. Producción Agraria - Nekazaritza Ekoizpena Saila
    • Artículos de revista DPA - NES Aldizkari artikuluak
    • View Item
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • Dpto. Producción Agraria - Nekazaritza Ekoizpena Saila
    • Artículos de revista DPA - NES Aldizkari artikuluak
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures

    Thumbnail
    View/Open
    20_Castanera_TranscriptionalEnzymatic.pdf (928.9Kb)
    20_MatCompl.zip (49.84Kb)
    Date
    2012
    Author
    Castanera Andrés, Raúl Upna
    Pérez Garrido, Gumersinda Upna
    Omarini, Alejandra 
    Alfaro Sánchez, Manuel Upna
    Pisabarro de Lucas, Gerardo Upna
    Faraco, Vicenza 
    Amore, Antonella 
    Ramírez Nasto, Lucía Upna
    Version
    Acceso abierto / Sarbide irekia
    xmlui.dri2xhtml.METS-1.0.item-type
    Artículo / Artikulua
    Version
    Versión publicada / Argitaratu den bertsioa
    Impact
     
     
     
    10.1128/aem.07880-11
     
     
     
    Show full item record
    Abstract
    The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitati ... [++]
    The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors. [--]
    Subject
    Pleurotus ostreatus, Submerged fermentation, Solid fermentation, Laccase gene transcription
     
    Publisher
    American Society for Microbiology
    Published in
    Applied and Environmental Microbiology, June 2012 Volume 78 Number 11, p. 4037–4045
    Departament
    Universidad Pública de Navarra. Departamento de Producción Agraria / Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
     
    Publisher version
    https://doi.org/10.1128/aem.07880-11
    URI
    https://hdl.handle.net/2454/31946
    Sponsorship
    This work was supported by funds from project AGL2008-05608-C02-01 of the Spanish National Research Plan, from Bioethanol-Euroinnova, and from the Autonomous Government of Navarre and by additional institutional support from the Public University of Navarre.
    Appears in Collections
    • Artículos de revista - Aldizkari artikuluak [2947]
    • Artículos de revista DPA - NES Aldizkari artikuluak [126]
    Items in Academica-e are protected by copyright with all rights reserved, unless otherwise noted.

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback
     

     

    Browse

    All of Academica-eCommunities & CollectionsAuthorsAuthors By Issue DateTitlesSubjectsBy DegreeThis CollectionAuthorsAuthors By Issue DateTitlesSubjectsBy Degree

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback