SarA positively controls Bap-dependent biofilm formation in Staphylococcus aureus
Fecha
2005Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/jb.187.16.5790-5798.2005
Resumen
The biofilm-associated protein Bap is a staphylococcal surface protein involved in biofilm formation. We
investigated the influence of the global regulatory locus sarA on bap expression and Bap-dependent biofilm
formation in three unrelated Staphylococcus aureus strains. The results showed that Bap-dependent biofilm
formation was diminished in the sarA mutants by an agr-independent mechanism. ...
[++]
The biofilm-associated protein Bap is a staphylococcal surface protein involved in biofilm formation. We
investigated the influence of the global regulatory locus sarA on bap expression and Bap-dependent biofilm
formation in three unrelated Staphylococcus aureus strains. The results showed that Bap-dependent biofilm
formation was diminished in the sarA mutants by an agr-independent mechanism. Complementation studies
using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation. As expected,
the diminished capacity to form biofilms in the sarA mutants correlated with the decreased presence of Bap in
the bacterial surface. Using transcriptional fusion and Northern analysis data, we demonstrated that the sarA
gene product acts as an activator of bap expression. Finally, the bap promoter was characterized and the
transcriptional start point was mapped by the rapid amplification of cDNA ends technique. As expected, we
showed that purified SarA protein binds specifically to the bap promoter, as determined by gel shift and DNase
I footprinting assays. Based on the previous studies of others as well as our work demonstrating the role for
SarA in icaADBC and bap expression (J. Valle, A. Toledo-Arana, C. Berasain, J. M. Ghigo, B. Amorena, J. R.
Penades, and I. Lasa, Mol. Microbiol. 48:1075–1087), we propose that SarA is an essential regulator controlling
biofilm formation in S. aureus. [--]
Materias
Staphylococcus aureus,
Bap-dependent biofilm formation,
sarA
Editor
American Society for Microbiology
Publicado en
Journal of Bacteriology, Aug. 2005, Vol. 187, No. 16, p. 5790–5798
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by grant BIO2002-04542-C02-01 from the
Comisión Interministerial de Ciencia y Tecnología (C.I.C.Y.T.) as well
as grants from the Cardenal Herrera-CEU University, from the Conselleria
de Agricultura, Pesca i Alimentació (CAPiA), and from the
Generalitat Valenciana (CTIDIA/2002/62, CTESPP/2003/027 and
AE04-8) to J.R.P., as well as grants from the NIH (AI37142 and
AI47441) to A.L.C. Fellowship support for M. Pilar Trotonda from the
Cardenal Herrera-CEU University is gratefully acknowledged.