Superinfection exclusion in alphabaculovirus infections is concomitant with actin reorganization
Fecha
2014Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1128/jvi.02974-13
Resumen
Superinfection exclusion is the ability of an established virus to interfere with a second virus infection. This effect was studied in
vitro during lepidopteran-specific nucleopolyhedrovirus (genus Alphabaculovirus, family Baculoviridae) infection. Homologous
interference was detected in Sf9 cells sequentially infected with two genotypes of Autographa californica multiple nucleopolyhedrovirus
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Superinfection exclusion is the ability of an established virus to interfere with a second virus infection. This effect was studied in
vitro during lepidopteran-specific nucleopolyhedrovirus (genus Alphabaculovirus, family Baculoviridae) infection. Homologous
interference was detected in Sf9 cells sequentially infected with two genotypes of Autographa californica multiple nucleopolyhedrovirus
(AcMNPV), each one expressing a different fluorescent protein. This was a progressive process in which a sharp decrease
in the signs of infection caused by the second virus was observed, affecting not only the number of coinfected cells observed,
but also the level of protein expression due to the second virus infection. Superinfection exclusion was concurrent with
reorganization of cytoplasmic actin to F-actin in the nucleus, followed by budded virus production (16 to 20 h postinfection).
Disruption of actin filaments by cell treatment with cytochalasin D resulted in a successful second infection. Protection against
heterologous nucleopolyhedrovirus infection was also demonstrated, as productive infection of Sf9 cells by Spodoptera frugiperda
nucleopolyhedrovirus (SfMNPV) was inhibited by prior infection with AcMNPV, and vice versa. Finally, coinfected cells
were observed following inoculation with mixtures of these two phylogenetically distant nucleopolyhedroviruses—AcMNPV
and SfMNPV—but at a frequency lower than predicted, suggesting interspecific virus interference during infection or replication.
The temporal window of infection is likely necessary to maintain genotypic diversity that favors virus survival but also permits
dual infection by heterospecific alphabaculoviruses. [--]
Materias
Superinfection exclusion,
Nucleopolyhedroviruses,
Alphabaculovirus infections,
Actin reorganization
Editor
American Society for Microbiology
Publicado en
Journal of Virology, March 2014 Volume 88 Number 6, p. 3548–3556
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
I.B. received a CSIC studentship. This research was supported by the
project AGL2011-30352-CO2-01 (Universidad Pública de Navarra, Pamplona,
Spain) and Oxford Brookes University, Oxford, United Kingdom.