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    Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes

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    Date
    2004
    Author
    Simón de Goñi, Oihane 
    Williams, Trevor Upna
    López Ferber, Miguel 
    Caballero Murillo, Primitivo Upna
    Version
    Acceso abierto / Sarbide irekia
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    Artículo / Artikulua
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    Versión publicada / Argitaratu den bertsioa
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    10.1128/aem.70.9.5579-5588.2004
     
     
     
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    Abstract
    A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant SfNIC-B was selected as the standard because its restriction profile corresponded to that of the wild-type isolate. Physical maps were generated for each of the variants. The ... [++]
    A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant SfNIC-B was selected as the standard because its restriction profile corresponded to that of the wild-type isolate. Physical maps were generated for each of the variants. The differences between variants and the SfNIC-B standard were confined to the region between map units 9 and 32.5. This region included PstI-G, PstI-F, PstI-L, PstI-K and EcoRI-L fragments. Eight genotypes presented a deletion in their genome compared with SfNIC-B. Occlusion body-derived virions of SfNIC-C, -D and -G accounted for 41% of plaque-purified clones. These variants were not infectious per os but retained infectivity by injection into S. frugiperda larvae. Median 50% lethal concentration values for the other cloned genotypes were significantly higher than that of the wild type. The variants also differed in their speed of kill. Noninfectious variants SfNIC-C and -D lacked the pif and pif-2 genes. Infectivity was restored to these variants by plasmid rescue with a plasmid comprising both pif and pif-2. Transcription of an SfNIC-G gene was detected by reverse transcription-PCR in insects, but no fatal disease developed. Transcription was not detected in SfNIC-C or -D-inoculated larvae. We conclude that the SfNIC population presents high levels of genetic diversity, localized to a 17-kb region containing pif and pif-2, and that interactions among complete and deleted genotypic variants will likely influence the capacity of this virus to control insect pests. [--]
    Subject
    Spodoptera frugiperda Nucleopolyhedrovirus Population
    Publisher
    American Society for Microbiology
    Published in
    Applied and Environmental Microbiology, vol. 70, nº 9, sept. 2004, p. 5579–5588
    Departament
    Universidad Pública de Navarra. Departamento de Producción Agraria / Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
     
    Publisher version
    https://doi.org/10.1128/aem.70.9.5579-5588.2004
    URI
    https://hdl.handle.net/2454/32070
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    • Artículos de revista DPA - NES Aldizkari artikuluak [115]
    • Artículos de revista - Aldizkari artikuluak [2168]
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