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dc.creatorValle Turrillas, Jaionees_ES
dc.creatorVergara Irigaray, Martaes_ES
dc.creatorMerino, Nekanees_ES
dc.creatorPenadés, José R.es_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.date.accessioned2019-01-23T11:16:23Z
dc.date.available2019-01-23T11:16:23Z
dc.date.issued2007
dc.identifier.issn0021-9193(Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32089
dc.description.abstractBiofilm formation in Staphylococcus aureus is subject to phase variation, and biofilm-negative derivatives emerge sporadically from a biofilm-positive bacterial population. To date, the only known mechanism for generating biofilm phenotypic variation in staphylococci is the reversible insertion/excision of IS256 in biofilm-essential genes. In this study, we present evidence suggesting that the absence of the σB transcription factor dramatically increases the rate of switching to the biofilm-negative phenotype in the clinical isolate S. aureus 15981, under both steady-state and flow conditions. The phenotypic switching correlates with a dramatic increase in the number of IS256 copies in the chromosomes of biofilm-negative variants, as well as with an augmented IS256 insertion frequency into the icaC and the sarA genes. IS256-mediated biofilm switching is reversible, and biofilm-positive variants could emerge from biofilm-negative σB mutants. Analysis of the chromosomal insertion frequency using a recombinant IS256 element tagged with an erythromycin marker showed an almost three-times-higher transposition frequency in a ΔσB strain. However, regulation of IS256 activity by σB appears to be indirect, since transposase transcription is not affected in the absence of σB and IS256 activity is inhibited to wild-type levels in a ΔσB strain under NaCl stress. Overall, our results identify a new role for σB as a negative regulator of insertion sequence transposition and support the idea that deregulation of IS256 activity abrogates biofilm formation capacity in S. aureus.en
dc.description.sponsorshipMarta Vergara-Irigaray is a predoctoral fellow (FPU) from the Ministerio de Educación y Ciencia, Spain. Nekane Merino is a predoctoral fellow from the Basque Government, Spain. This work was supported by the BIO2005-08399 grant from the Spanish Ministerio de Educación y Ciencia and grant LSHM-CT-2006-019064 from the European Union.en
dc.format.extent11 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, vol. 189, nº 7, apr. 2007, p. 2886–2896en
dc.rights© 2007, American Society for Microbiology. All Rights Reserved.en
dc.subjectBiofilm phenotypic variationen
dc.titleB regulates IS256-mediated Staphylococcus aureus biofilm phenotypic variationen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jb.01767-06
dc.relation.publisherversionhttps://doi.org/10.1128/jb.01767-06
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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