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dc.creatorAguilera, Selenees_ES
dc.creatorLopez-Lopez, K.es_ES
dc.creatorNieto, Y.es_ES
dc.creatorGarciduenas-Pina, R.es_ES
dc.creatorHernandez-Guzman, G.es_ES
dc.creatorHernandez-Flores, J.es_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorÁlvarez Morales, Arieles_ES
dc.date.accessioned2019-01-23T11:16:24Z
dc.date.available2019-01-23T11:16:24Z
dc.date.issued2007
dc.identifier.issn0021-9193(Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32091
dc.description.abstractPseudomonas syringae pv. phaseolicola is the causal agent of halo blight disease of beans (Phaseolus vulgaris L.), which is characterized by water-soaked lesions surrounded by a chlorotic halo resulting from the action of a non-host-specific toxin known as phaseolotoxin. This phytotoxin inhibits the enzyme ornithine carbamoyltransferase involved in arginine biosynthesis. Different evidence suggested that genes involved in phaseolotoxin production were clustered. Two genes had been previously identified in our laboratory within this cluster: argK, which is involved in the immunity of the bacterium to its own toxin, and amtA, which is involved in the synthesis of homoarginine. We sequenced the region around argK and amtA in P. syringae pv. phaseolicola NPS3121 to determine the limits of the putative phaseolotoxin gene cluster and to determine the transcriptional pattern of the genes comprising it. We report that the phaseolotoxin cluster (Pht cluster) is composed of 23 genes and is flanked by insertion sequences and transposases. The mutation of 14 of the genes within the cluster lead to a Tox− phenotype for 11 of them, while three mutants exhibited low levels of toxin production. The analysis of fusions of selected DNA fragments to uidA, Northern probing, and reverse transcription-PCR indicate the presence of five transcriptional units, two monocistronic and three polycistronic; one is internal to a larger operon. The site for transcription initiation has been determined for each promoter, and the putative promoter regions were identified. Preliminary results also indicate that the gene product of phtL is involved in the regulation of the synthesis of phaseolotoxin.en
dc.description.sponsorshipThe work reported was funded by grants from CONACYT to A. Alvarez-Morales (Research grant) and S. Aguilera (graduate student scholarship) and from the Ministerio de Educación y Ciencia (AGL2004-03143) to J. Murillo.en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, vol. 189, nº 7, apr. 2007, p. 2834–2843en
dc.rights© 2007, American Society for Microbiology. All Rights Reserved.en
dc.subjectPseudomonas syringae pv. Phaseolicolaen
dc.titleFunctional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxinen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jb.01845-06
dc.relation.publisherversionhttps://doi.org/10.1128/jb.01845-06
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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