Tryptophan levels as a marker of auxins and nitric oxide signaling
Fecha
2022Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-52396-P
Impacto
|
10.3390/plants1110130
Resumen
The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA),
which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of
the most common intermediates. Auxin signaling is well known to interact with free radical nitric
oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and
nitrogen nutrit ...
[++]
The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA),
which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of
the most common intermediates. Auxin signaling is well known to interact with free radical nitric
oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and
nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to
analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after
the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude
extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed
by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal
component analysis (PCA) was performed on N-metabolism-related compounds, as classified by
the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls
and treatments are mainly explained by the differences in Trp contents, which are much higher in
controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing
some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass-
identified compounds between GSNO- and IAOx-treated cells are mainly explained by their
concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic
acid, which is related to glutathione. These results show that the contents in Trp can be a marker for
the study of auxin and NO signaling. [--]
Materias
Tryptophan,
Indole-3-acetaldoxime,
IAOx,
Nitric oxide,
NO,
Stress,
Principal-component analysis
Editor
MDPI
Publicado en
Plants, 2022, 11 (10),1304-1304
Departamento
Universidad Pública de Navarra. Departamento de Ciencias /
Nafarroako Unibertsitate Publikoa. Zientziak Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Advanced Materials and Mathematics - INAMAT2 /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This work was supported by grants from the Spanish Ministry of Science and Innovation
(AGL2017-52396-P, co-financed by the European Regional Development Fund, FEDER) and a grant
from the Public University of Navarre (Res 309/2022) to J.F.M. M.U. is a recipient of a pre-doctoral
fellowship from the Government of Navarre, Spain. J.B. and P.L. have received pre-doctoral fellow-
ships from the Public University of Navarre, Spain. J.B. was supported by the Romanian Ministry of
Education and research, grant CNCS-UEFISCDI, project number PN-III-P4-ID-PCE-2020-2696, within
PNCDI III.