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Moleres Apilluelo, Javier

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https://academica-e.unavarra.es/handle/2454/49598

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Moleres Apilluelo

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Javier

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Instituto de Agrobiotecnología (IdAB)

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0000-0002-2749-6988

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810165

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2015 - 20182
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Subject: Haemophilus parasuis ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs
    (American Society for Microbiology, 2015) Moleres Apilluelo, Javier; Santos López, Alfonso; Lázaro, Isidro; Labairu, Javier; Prat, Cristina; Ardanuy, Carmen; González Zorn, Bruno; Aragon, Virginia; Garmendia García, Juncal; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Haemophilus parasuis, the causative agent of Glässer’s disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer’s disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria.
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    PublicationOpen Access
    Patho-adaptive evolution of Haemophilus spp. bacterial respiratory colonizing opportunistic pathogens
    (2018) Moleres Apilluelo, Javier; Garmendia García, Juncal; Producción Agraria; Nekazaritza Ekoizpena
    Esta tesis doctoral se ha centrado en tres aspectos de la pato-adaptación de dos especies bacterianas relacionadas filogenéticamente, que colonizan de forma asintomática y también son patógenos oportunistas en los sistemas respiratorios porcino y humano, respectivamente. Para ello, hemos analizado rasgos de evolución patoadaptativa bacteriana en un modelo de trabajo experimental (Capítulo 2) y de forma natural dentro del hospedador (Capítulos 1 y 3).