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Orcaray Echeverría, Luis

Last Name

Orcaray Echeverría

First Name

Luis

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Ciencias del Medio Natural

ORCID

0000-0002-9832-1448

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6182

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Now showing 1 - 9 of 9

Open Access
The pattern of shikimate pathway and phenylpropanoids after inhibition by glyphosate or quinate feeding in pea roots
(Elsevier, 2017) Zabalza Aznárez, Ana; Orcaray Echeverría, Luis; Fernández Escalada, Manuel; Zulet González, Ainhoa; Royuela Hernando, Mercedes; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
The shikimate pathway is a metabolic route for the biosynthesis of aromatic amino acids (AAAs) (i.e. phenylalanine, tyrosine, and tryptophan). A key enzyme of shikimate pathway (5-enolpyruvylshikimate-3-phosphate synthase, EPSPS) is the target of the widely used herbicide glyphosate. Quinate is a compound synthesized in plants through a side branch of the shikimate pathway. Glyphosate provokes quinate accumulation and exogenous quinate application to plants shows a potential role of quinate in the toxicity of the herbicide glyphosate. Based on this, we hypothesized that the role of quinate accumulation in the toxicity of the glyphosate would be mediated by a deregulation of the shikimate pathway. In this study the effect of the glyphosate and of the exogenous quinate was evaluated in roots of pea plants by analyzing the time course of a full metabolic map of several metabolites of shikimate and phenylpropanoid pathways. Glyphosate application induced an increase of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS, first enzyme of the shikimate pathway) protein and accumulation of metabolites upstream of the enzyme EPSPS. No common effects on the metabolites and regulation of shikimate pathway were detected between quinate and glyphosate treatments, supporting that the importance of quinate in the mode of action of glyphosate is not mediated by a common alteration of the regulation of the shikimate pathway. Contrary to glyphosate, the exogenous quinate supplied was probably incorporated into the main trunk from the branch pathway and accumulated in the final products, such as lignin, concomitant with a decrease in the amount of DAHPS protein.
Open Access
Tillage effects on soil quality after three years of irrigation in Northern Spain
(MDPI, 2017) Apesteguía Barberena, Marcos; Virto Quecedo, Íñigo; Orcaray Echeverría, Luis; Bescansa Miquel, Paloma; Enrique Martín, Alberto; Imaz Gurruchaga, María José; Karlen, Douglas; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
Irrigation is being initiated on large areas of traditionally rainfed land to meet increasing global demand for food, feed, fiber and fuel. However, the consequences of this transition on soil quality (SQ) have scarcely been studied. Therefore, after previously identifying the most tillage-sensitive SQ indicators under long-term rainfed conditions, conversion of a research site on a Haplic Calcisol in Navarre, in northeast Spain provided an ideal location to reevaluate those SQ indicators after three years of irrigated management. The Soil Management Assessment Framework (SMAF) was used to test our hypothesis that adopting irrigation could change the sensitivity and importance of non-irrigated SQ indicators. Several soil physical, chemical, and biological indicators along with crop yields were used to evaluate SQ three years after initiating irrigation on a long-term conventional tillage (CT), minimum tillage (MT) and no-tillage (NT) study where either barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.) was being grown. The results confirmed our hypothesis that irrigation would change the relative importance of various SQ indicators and suggested that some SMAF algorithms, such as those used to assess bulk density, needed to be recalibrated for these Mediterranean soils.
Open Access
Soil quality assessment after 25 years of sewage sludge vs. mineral fertilization in a calcareous soil
(MDPI, 2021) Simoes da Mota, Ana Claudia; Poch, Rosa María; Enrique Martín, Alberto; Orcaray Echeverría, Luis; Virto Quecedo, Íñigo; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
The aim of this work was to identify the most sensitive soil quality indicators and assess soil quality after long-term application of sewage sludge (SS) and conventional mineral fertilization for rainfed cereal production in a sub-humid Mediterranean calcareous soil. The treatments included six combinations of SS at different doses (40 t ha−1 and 80 ha−1) and frequencies (every 1, 2 and 4 years), plus a control with mineral fertilization, and a baseline control without fertilization. Twenty-five years after the onset of the experiment, 37 pre-selected physical, chemical and biological soil parameters were measured, and a minimum data set was determined. Among these indicators, those significantly affected by treatment and depth were selected as sensitive. A principal component analysis (PCA) was then performed for each studied depth. At 0–15 cm, PCA identified three factors (F1, F2 and F3), and at 15–30 cm, two factors (F4 and F5) that explained 71.5% and 67.4% of the variation, respectively, in the soil parameters. The most sensitive indicators (those with the highest correlation within each factor) were related to nutrients (P and N), organic matter, and trace metals (F1 and F4), microporosity (F2), earthworm activity (F3), and exchangeable cations (F5). Only F3 correlated significantly (and negatively) with yield. From these results, we concluded that soil quality can be affected in opposite directions by SS application, and that a holistic approach is needed to better assess soil functioning under SS fertilization in this type of agrosystem.
Open Access
Impairment of carbon metabolism induced by the herbicide glyphosate
(Elsevier, 2012) Orcaray Echeverría, Luis; Zulet González, Amaia; Zabalza Aznárez, Ana; Royuela Hernando, Mercedes; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
The herbicide glyphosate reduces plant growth and causes plant death by inhibiting the biosynthesis of aromatic amino acids. The objective of this work was to determine whether glyphosate-treated plants show a carbon metabolism pattern comparable to that of plants treated with herbicides that inhibit branched-chain amino acid biosynthesis. Glyphosate-treated plants showed impaired carbon metabolism with an accumulation of carbohydrates in the leaves and roots. The growth inhibition detected after glyphosate treatment suggested impaired metabolism that impedes the utilization of available carbohydrates or energy at the expected rate. These effects were common to both types of amino acid biosynthesis inhibitors. Under aerobic conditions, ethanolic fermentative metabolism was enhanced in the roots of glyphosate-treated plants. This fermentative response was not related to changes in the respiratory rate or to a limitation of the energy charge. This response, which was similar for both types of herbicides, might be considered a general response to stress conditions.
Open Access
Soil water retention and soil compaction assessment in a regional-scale strategy to improve climate change adaptation of agriculture in Navarre, Spain
(MDPI, 2021) Antón Sobejano, Rodrigo; Ruiz Sagaseta de Ilurdoz, Alberto; Orcaray Echeverría, Luis; Arricibita Videgain, Javier; Enrique Martín, Alberto; De Soto García, I. S.; Virto Quecedo, Íñigo; Zientziak; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Ciencias; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
The aim of this study was to evaluate the effectiveness of the different agricultural management adaptive strategies considered in the framework of a regional climate change adaptation roadmap in Navarre (Spain), from the point of view of soil physical indicators associated to soil compaction and water retention. These indicators were chosen as representative of the potential of these strategies to improve the soil physical condition. That for, the effectiveness of conservation agriculture (CA), crop rotations (ROT), additions of organic matter (ExO), irrigation (IRR) and innovative grassland management (GSS) was assessed by monitoring soil bulk density (BD) and soil available water holding capacity (AWHC) in a network of 159 agricultural fields across homogeneous agro-climatic zones in the region. A sampling protocol designed to compare groups of plots with or without adaptive practices, and with equal soil characteristics within each zone, allowed to determine the effect size of each strategy (measured as response ratios, RR, calculated as the relative value of BD and AWHC in fields with adaptive management vs. without). Both parameters responded to soil and crop management, although the observed effect was highly variable. Only the ExO strategy showed an overall positive effect on BD. ROT, IRR and GSS displayed no effect and, in the case of CA, the effect was negative. In terms of AWHC, although the results within the zones were heterogeneous, the overall effect associated to the strategies ROT, ExO, IRR and GSS was neutral, and only CA resulted in an overall negative effect. The observed variability in terms of the effectiveness of the five strategies tested in this region highlights the need to understand the complexity of interrelationships between management and dynamic soil properties at the regional scale.
Open Access
Cover crops in viticulture. A systematic review (2): Implications on vineyard agronomic performance
(International Viticulture and Enology Society (IVES), 2021) Abad Zamora, Francisco Javier; Hermoso de Mendoza, Irantzu; Marín Ederra, Diana; Orcaray Echeverría, Luis; Santesteban García, Gonzaga; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura
The present systematic review aims to provide an overview of the impact of cover crops on vegetative growth and the productive parameters of vineyards. A systematic review was made on Scopus-index journals dating from 1999 to 2018. The selection was made at the same time by two different researchers, who selected a total of 272 published papers related to cover crops in vineyards. Each article was categorised according to its theme and a metadata database was created, considering all relevant information from an agronomic point of view for each article. It can be concluded from the review that the use of cover crops can reduce vine vegetative growth, which in turn can help keep the incidence of fungal diseases (like grey mould) at a low level. In general, this practice does not have a clear effect on vineyard yield or grape juice parameters, like total soluble solids (TSS) or titratable acidity (TA). Cover crops can decrease vineyard pests to a certain extent, especially Cicadellidae. Cover crops can sometimes sporadically cause water stress in the vineyard, but only during the summer months. This review allowed us to summarise available information on cover crops and their effects on vineyard agronomic performance in a systematic way. Such information can be used to help select the most suitable cover, based on specific vineyard objectives and growing conditions.
Open Access
A fertilisation strategy combining mineral fertiliser and biosolid improves long-term yield and carbon storage in a calcareous soil
(MDPI, 2023) Zaragüeta, Armelle; Enrique Martín, Alberto; Portell Canal, Xavier; Antón Sobejano, Rodrigo; Virto Quecedo, Íñigo; Orcaray Echeverría, Luis; Ciencias; Zientziak; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
At a strategic moment for agricultural soils, which are expected to contribute to climate change mitigation through carbon storage while safely feeding a growing world population, the fertiliser strategies used will be key. In a calcareous soil with extensive rainfed agricultural use and straw removal, different fertiliser strategies were evaluated with the aim of determining their effects on crop yield, nitrogen agronomic efficiency, and the storage of organic carbon and total nitrogen in the soil. Different doses of mineral fertiliser, expressed as kg of mineral nitrogen ha−1 year−1 (0, 60, 120, 180, and 240 nitrogen fertilising units (NFUs)), were applied to plots with and without biosolid amendment. The biosolid, applied at a rate of 40 Mg ha−1 every 3 years for 18 years, complied with national and European regulations to be applied on agricultural soil. The use of combined fertilisation reduced the amount of mineral fertiliser applied between 33 and 67% and the total fertiliser units between 7 and 40%, while maintaining similar yields to the reference mineral fertilisation (180 NFUs). These results could be related to a higher nitrogen agronomic efficiency in the combined fertilisation treatments that do not exceed the total NFUs required by the crop. Combined fertilisation was also an effective fertiliser technique to store total nitrogen and organic carbon in the soil. However, compared to the reference mineral fertilisation (180 NFUs), no significant changes in the soil organic carbon were observed, probably due to the crop management method in which the straw is removed and to higher gas emissions. Our results support the need to assess the efficacy of each agricultural technique at local scales in order not to overestimate or underestimate the potential of each agricultural technique to store soil organic carbon.
Open Access
Efecto de la agricultura de conservación y la fertilización nitrogenada en la población de plantas adventicias en una parcela de trigo en regadío
(Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2017) Arias Fariñas, Nerea; Lezáun, Juan Antonio; Garnica, Irache; Orcaray Echeverría, Luis; Pérez de Ciriza, José Jesús; Delgado, Javier
El uso de técnicas como la agricultura de conservación o la reducción de nitrógeno aplicado en campo pueden causar cambios en la composición y abundancia de las especies de malas hierbas presentes en los sistemas de cultivo. En el monitoreo de la flora adventicia en un cultivo de trigo que siguió una rotación de maíz-sorgo-trigo se observó que especies como Avena sterilis o Salsola kali desaparecieron tras la rotación de dos cultivos de verano. La aplicación de una dosis de fertilización nitrogenada reducida no produjo diferencias significativas en el número de plantas presentes por metro cuadrado en las parcelas de estudio. Sin embargo, la aplicación del no laboreo (NL) generó un mayor desarrollo de la biomasa de las plantas adventicias lo que puede conducir a una mayor competencia con el cultivo, y por ello, una disminución del rendimiento.
Open Access
Soil organic carbon monitoring to assess agricultural climate change adaptation practices in Navarre, Spain
(Springer, 2021) Antón Sobejano, Rodrigo; Arricibita Videgain, Javier; Ruiz Sagaseta de Ilurdoz, Alberto; Enrique Martín, Alberto; De Soto García, I. S.; Orcaray Echeverría, Luis; Zaragüeta, Armelle; Virto Quecedo, Íñigo; Zientziak; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Ciencias; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Climate change adaptation strategies are needed for agriculture, one of the most vulnerable human activities. In Navarre, North of Spain, ongoing adaptive management practices were identified and promoted in the framework of a regional adaptation strategy. Most include practices aiming to increase topsoil organic carbon (SOC) in agricultural land. In this work, the effectiveness of these practices (conservation agriculture, crop rotations, additions of organic matter, irrigation, and controlled grazing management) was assessed by means of monitoring SOC in a network of 159 agricultural fields across the region. These fields were selected across bioclimatic zones, where soil vulnerabilities and land-uses were previously assessed, to represent the most widespread conditions in the region. A sampling protocol designed to compare SOC stocks in plots with equal soil conditions within each zone, and with or without adaptive practices, allowed the determination of their effect size (measured as response ratios, RR). Exogenous organic matter addition was the most effective practice for SOC storage (RR 95% confidence interval (CI) [1.25–1.37]) across the region. Controlled grazing also resulted in net SOC gains (RR CI [1.13–1.42]) in temperate and semiarid grasslands. Conservation agriculture seemed to be more effective in the driest zone (RR CI [1.30–1.53]) than in the more humid ones (RR CI [0.98–1.21]). Irrigation also displayed a net positive effect (RR CI [1.17–1.34]), modulated by irrigated crop management, whereas crop rotations had an overall negative impact vs. monoculture (RR CI [0.84–0.96]), likely by their interaction with irrigation. These results confirm the variability in SOC responses to changes in management, and SOC as an indicator for assessing regional adaptation practices, although other biophysical, agronomic, and socio-economic factors also need to be accounted for.