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Martínez Bilbao, Alejandro

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Martínez Bilbao

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Alejandro

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Producción Agraria

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2011 - 20122
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Author: Murillo Martínez, Jesús ×

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    PublicationOpen Access
    Miniature transposable sequences are frequently mobilized in the bacterial plant pathogen Pseudomonas syringae pv. phaseolicola
    (Public Library of Science, 2011) Bardají Goikoetxea, Leire; Añorga García, Maite; Jackson, Robert W.; Martínez Bilbao, Alejandro; Yanguas Casas, Natalia; Murillo Martínez, Jesús; Producción Agraria; Nekazaritza Ekoizpena
    Mobile genetic elements are widespread in Pseudomonas syringae, and often associate with virulence genes. Genome reannotation of the model bean pathogen P. syringae pv. phaseolicola 1448A identified seventeen types of insertion sequences and two miniature inverted-repeat transposable elements (MITEs) with a biased distribution, representing 2.8% of the chromosome, 25.8% of the 132-kb virulence plasmid and 2.7% of the 52-kb plasmid. Employing an entrapment vector containing sacB, we estimated that transposition frequency oscillated between 2.6 x 10(-5) and 1.1 x 10(-6), depending on the clone, although it was stable for each clone after consecutive transfers in culture media. Transposition frequency was similar for bacteria grown in rich or minimal media, and from cells recovered from compatible and incompatible plant hosts, indicating that growth conditions do not influence transposition in strain 1448A. Most of the entrapped insertions contained a full-length IS801 element, with the remaining insertions corresponding to sequences smaller than any transposable element identified in strain 1448A, and collectively identified as miniature sequences. From these, fragments of 229, 360 and 679-nt of the right end of IS801 ended in a consensus tetranucleotide and likely resulted from one-ended transposition of IS801. An average 0.7% of the insertions analyzed consisted of IS801 carrying a fragment of variable size from gene PSPPH_0008/PSPPH_0017, showing that IS801 can mobilize DNA in vivo. Retrospective analysis of complete plasmids and genomes of P. syringae suggests, however, that most fragments of IS801 are likely the result of reorganizations rather than one-ended transpositions, and that this element might preferentially contribute to genome flexibility by generating homologous regions of recombination. A further miniature sequence previously found to affect host range specificity and virulence, designated MITEPsy1 (100-nt), represented an average 2.4% of the total number of insertions entrapped in sacB, demonstrating for the first time the mobilization of a MITE in bacteria.
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    PublicationOpen Access
    Venturia inaequalis resistance in local Spanish cider apple germplasm under controlled and field conditions
    (Springer Netherlands, 2012) Martínez Bilbao, Alejandro; Ortiz Barredo, Amaia; Montesinos, Emilio; Murillo Martínez, Jesús; Producción Agraria; Nekazaritza Ekoizpena; Gobierno de Navarra / Nafarroako Gobernua: PA123
    Host resistance is a key method for the integrated management of apple scab caused by Venturia inaequalis, which is one of the most important diseases of apple. Artificial inoculation of 92 cider apple cultivars with a mixed inoculum of V. inaequalis identified 19 weakly resistant and 19 resistant cultivars. Twelve of these resistant cultivars were previously classified as having low susceptibility to fire blight, and four of them showed complete or weak resistance to races (1), (1, 6) and (6, 7, 13) of V. inaequalis. The analysis of a selection of 72 cultivars for 6 years under field conditions identified 14 cultivars that were classified as resistant to apple scab under high disease pressure involving one to six Mills periods of severe risk of infection each year. Eight out of these 14 cultivars previously showed high levels of resistance to fire blight, which would allow the incorporation of genetic resistance in the integrated production of cider apples in Spain through their use in breeding programs.