Barajas Vélez, Miguel Ángel

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Barajas Vélez

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Miguel Ángel

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Ciencias de la Salud

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IMAB. Research Institute for Multidisciplinary Applied Biology

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  • PublicationOpen Access
    Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue
    (Elsevier, 2016) Zapata Linares, Natalia; Rodríguez, Saray; Mazo, Manuel; Abizanda, Gloria; Barajas Vélez, Miguel Ángel; Ciencias de la Salud; Osasun Zientziak
    In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.