Person:
Serrano Mendioroz, Irantzu

Profile Picture

Email Address

person.page.identifierURI

https://academica-e.unavarra.es/handle/2454/50029

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Serrano Mendioroz

First Name

Irantzu

person.page.departamento

ProducciĆ³n Agraria

person.page.instituteName

ORCID

person.page.upna

9069

Name

Filters

20111
Reset filters

Settings

Has files: true Ɨ Author: Celay Leoz, Ion Ɨ Author: Serrano Mendioroz, Irantzu Ɨ Subject: Gene regulation Ɨ Subject: HSD17Bs Ɨ Item Type: Publication Ɨ Type: info:eu-repo/semantics/article Ɨ

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    PublicationOpen Access
    Transcriptional regulation of type 11 17Ī²-hydroxysteroid dehydrogenase expression in prostate cancer cells
    (Elsevier, 2011) Rotinen DĆ­az, Mirja Sofia; Villar BĆ©cares, JoaquĆ­n; Celay Leoz, Ion; Serrano Mendioroz, Irantzu; Notario, Vicente; EncĆ­o MartĆ­nez, Ignacio; Ciencias de la Salud; Osasun Zientziak; Gobierno de Navarra / Nafarroako Gobernua
    Type 11 Hydroxysteroid (17-beta) dehydrogenase (HSD17B11) catalyzes the conversion of 5Ī±-androstan-3Ī±,17Ī²-diol into androsterone suggesting that it may play an important role in androgen metabolism. We previously described that overexpression of C/EBPĪ± or C/EBPĪ² induced HSD17B11 expression in HepG2 cells but this process was not mediated by the CCAAT boxes located within its proximal promoter region. Here, we study HSD17B11 transcriptional regulation in prostate cancer (PC) cells. Transfection experiments showed that the region āˆ’107/+18 is sufficient for promoter activity in PC cells. Mutagenesis analysis indicated that Sp1 and C/EBP binding sites found in this region are essential for promoter activity. Additional experiments demonstrated that ectopic expression of Sp1 and C/EBPĪ± upregulated HSD17B11 expression only in PC cell lines. Through DAPA and ChIP assays, specific recruitment of Sp1 and C/EBPĪ± to the HSD17B11 promoter was detected. These results show that HSD17B11 transcription in PC cells is regulated by Sp1 and C/EBPĪ±.