Baslam, Marouane

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https://academica-e.unavarra.es/handle/2454/48678

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Baslam

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Marouane

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Instituto de Agrobiotecnología (IdAB)

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810937

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2018 - 201912020 - 20251
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Subject: Elevated CO2 ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Nitrogen fertilization form and energetic status as target points conditioning rice responsiveness to elevated [CO2]
    (Frontiers Media, 2025-03-11) Jáuregui Mosquera, Iván; Mitsui, Toshiaki; Gakière, Bertrand; Mauve, Caroline; Gilard, Françoise; Aranjuelo Michelena, Iker; Baslam, Marouane; Ciencias; Zientziak
    The nitrogen (N) fertilization form and plant energy status are known to significantly influence plant responses to elevated atmospheric carbon dioxide (CO2) concentrations. However, a close examination of the interplay between N sources under contrasting light intensity has been notably absent in the literature. In this study, we conducted a factorial experiment with rice plants involving two different light intensities (150 and 300 µmol m-2 s-1), inorganic N sources [nitrate (N-NO3) or ammonium nitrate (N-NH4NO3)] at varying CO2 levels (410 and 700 parts per million, ppm). The aim was to examine the individual and combined effects of these factors on the allocation of biomass in whole plants, as well as on leaf-level photosynthetic characteristics, chloroplast morphology and development, ATP content, ionomics, metabolomics, and hormone profiles. Our research hypothesis posits that mixed nutrition enhances plant responsiveness to elevated CO2 (eCO2) at both light levels compared to sole N-NO3 nutrition, due to its diminished energy demands for plant assimilation. Our findings indicate that N-NO3 nutrition does not promote the growth of rice, its photosynthetic capacity, or N content when exposed to ambient CO2 (aCO2), and is significantly reduced in low light (LL) conditions. Rice plants with N-NH4NO3 exhibited a higher carboxylation capacity, which resulted in larger biomass (total C, tiller number, and lower root-shoot ratio) supported by higher Calvin-cycle-related sugars. The lower leaf N content and overall amino acid levels at eCO2, particularly pronounced in N-NO3, combined with the lower ATP content (lowest at LL and N-NO3), may reflect the higher energy costs of N assimilation at eCO2. We also observed significant plasticity patterns in leaves under eCO2. Our findings highlight the importance of a thorough physiological understanding to inform innovative management practices aimed at mitigating the negative effects of climate change on plant N use efficiency.
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    PublicationOpen Access
    Proteomics analysis reveals non-controlled activation of photosynthesis and protein synthesis in a rice npp1 mutant under high temperature and elevated CO2 conditions
    (MDPI, 2018) Inomata, Takuya; Baslam, Marouane; Masui, Takahiro; Koshu, Tsutomu; Takamatsu, Takeshi; Kaneko, Kentaro; Pozueta Romero, Javier; Mitsui, Toshiaki; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Rice nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides including ADP-glucose and ATP. Under high temperature and elevated CO2 conditions (HT + ECO2), the npp1 knockout rice mutant displayed rapid growth and high starch content phenotypes, indicating that NPP1 exerts a negative effect on starch accumulation and growth. To gain further insight into the mechanisms involved in the NPP1 downregulation induced starch overaccumulation, in this study we conducted photosynthesis, leaf proteomic, and chloroplast phosphoproteomic analyses of wild-type (WT) and npp1 plants cultured under HT + ECO2. Photosynthesis in npp1 leaves was significantly higher than in WT. Additionally, npp1 leaves accumulated higher levels of sucrose than WT. The proteomic analyses revealed upregulation of proteins related to carbohydrate metabolism and the protein synthesis system in npp1 plants. Further, our data indicate the induction of 14-3-3 proteins in npp1 plants. Our finding demonstrates a higher level of protein phosphorylation in npp1 chloroplasts, which may play an important role in carbohydrate accumulation. Together, these results offer novel targets and provide additional insights into carbohydrate metabolism regulation under ambient and adverse conditions.