Ruiz de Escudero Fuentemilla, Íñigo

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Ruiz de Escudero Fuentemilla

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Íñigo

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Agronomía, Biotecnología y Alimentación

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IMAB. Research Institute for Multidisciplinary Applied Biology

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Now showing 1 - 2 of 2
  • PublicationOpen Access
    Potential of the Bacillus thuringiensis toxin reservoir for the control of Lobesia botrana (Lepidoptera: tortricidae), a major pest of grape plants
    (American Society for Microbiology, 2006) Ruiz de Escudero Fuentemilla, Íñigo; Estela, Anna; Escriche, Baltasar; Caballero Murillo, Primitivo; Producción Agraria; Nekazaritza Ekoizpena; Gobierno de Navarra / Nafarroako Gobernua, FP2000-5497
    The potential of Bacillus thuringiensis Cry proteins to control the grape pest Lobesia botrana was explored by testing first-instar larvae with Cry proteins belonging to the Cry1, Cry2, and Cry9 groups selected for their documented activities against Lepidoptera. Cry9Ca, a toxin from B. thuringiensis, was the protein most toxic to L. botrana larvae, followed in decreasing order by Cry2Ab, Cry1Ab, Cry2Aa, and Cry1Ia7, with 50% lethal concentration values of 0.09, 0.1, 1.4, 3.2, and 8.5 μg/ml of diet, respectively. In contrast, Cry1Fa and Cry1JA were not active at the assayed concentration (100 μg/ml). In vitro binding and competition experiments showed that none of the toxins tested (Cry1Ia, Cry2Aa, Cry2Ab, and Cry9C) shared binding sites with Cry1Ab. We conclude that either Cry1Ia or Cry9C could be used in combination with Cry1Ab to control this pest, either as the active components of B. thuringiensis sprays or expressed together in transgenic plants.
  • PublicationOpen Access
    Vip3C, a novel class of vegetative insecticidal proteins from Bacillus thuringiensis
    (American Society for Microbiology, 2012) Palma Dovis, Leopoldo; Hernández Rodríguez, C.; Maeztu Martínez, Mireya; Hernández Martínez, Patricia; Ruiz de Escudero Fuentemilla, Íñigo; Escriche, Baltasar; Muñoz Labiano, Delia; Rie, Jeroen van; Ferré, Juan; Caballero Murillo, Primitivo; Nekazaritza Ekoizpena; Producción Agraria; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Three vip3 genes were identified in two Bacillus thuringiensis Spanish collections. Sequence analysis revealed a novel Vip3 protein class (Vip3C). Preliminary bioassays of larvae from 10 different lepidopteran species indicated that Vip3Ca3 caused more than 70% mortality in four species after 10 days at 4 μg/cm2.