Person:
Sesma Pascual, María Teresa

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

Job Title

Last Name

Sesma Pascual

First Name

María Teresa

person.page.departamento

Producción Agraria

ORCID

person.page.upna

9030

Name

Filters

20101
Reset filters

Settings

Has files: true × Subject: Adenylate energy charge ×

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    PublicationOpen Access
    Genome-wide screening of genes whose enhanced expression affects glycogen accumulation in Escherichia coli
    (Oxford University Press, 2010) Eydallin, Gustavo; Montero Macarro, Manuel; Almagro Zabalza, Goizeder; Sesma Pascual, María Teresa; Viale, Alejandro M.; Muñoz Pérez, Francisco José; Rahimpour, Mehdi; Baroja Fernández, Edurne; Pozueta Romero, Javier; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.