Andrés Cara, Damián de

Profile Picture

Email Address

person.page.identifierURI

https://academica-e.unavarra.es/handle/2454/48531

Birth Date

Job Title

Last Name

Andrés Cara

First Name

Damián de

person.page.departamento

Instituto de Agrobiotecnología (IdAB)

person.page.instituteName

ORCID

0000-0001-7302-807X

person.page.observainves

person.page.upna

4002

Name

Filters

20062
Reset filters

Settings

End date: 2009 × Start date: 2006 ×

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    PublicationOpen Access
    Maedi-visna virus infection of ovine mammary epithelial cells
    (EDP Sciences, 2006) Bolea, Rosa; Monleón, Eva; Carrasco, Librado; Vargas, Antonia; Andrés Cara, Damián de; Amorena Zabalza, Beatriz; Badiola, Juan José; Luján, Lluís; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    The aim of this work was to perform a complete study of maedi-visna virus (MVV) infected mammary glands of naturally-infected sheep, and to determine if cells other than macrophages undergo a productive viral infection in this organ. Fifteen seropositive and two seronegative ewes were selected from MVV-infected flocks on the basis of clinical indurative mastitis and three sheep from an MVV-free flock. Within the mammary gland, MVV-positive cells were located by immunohistochemistry in the stroma and the epithelial alveolar barrier, most likely the ovine mammary epithelial cells (OMEC) of the acini. In situ hybridization confirmed these findings. Ultrastructural studies showed the presence of lentivirus-like particles budding off the cell surface in the alveolar barrier and also free in the acinar lumen. The presence of mammary histopathological lesions and MVV together with clear indications of productive infection (demonstration of a cytopathic effect in OMEC cultures and infection of co-cultures) were observed in the 15 seropositive and one of the seronegative sheep from the infected flock. These findings demonstrate that the OMEC were infected in vivo and probably underwent productive infection when studied ex-vivo. The OMEC of MVV-free sheep, which had subsequently been infected in vitro with MVV, also showed productive infection when challenged in vitro, confirming the replication of MVV in OMEC in vitro. The presence of MVV-infected OMEC in the mammary gland from infected animals, the productive infection in these OMEC and the release of lentiviral particles to the acinar lumen may have relevance in the pathogenesis and transmission of MVV infection.
  • Thumbnail Image
    PublicationOpen Access
    Extensive rearing hinders Maedi-Visna Virus (MVV) infection in sheep
    (EDP Sciences, 2006) Leginagoikoa, Iratxe; Juste, Ramón; Barandika, Jesse; Amorena Zabalza, Beatriz; Andrés Cara, Damián de; Luján, Lluís; Badiola, Juan José; Berriatua, Eduardo; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Maedi-Visna Virus (MVV) seroprevalence and its relationship with housing and mode of rearing of replacement ewe-lambs was investigated in 38 non-randomly selected sheep-flocks in Spain. They included extensive lamb-producing Manchega cross-bred flocks raised almost permanently at pasture, semi-intensive Latxa dairy flocks housed 2–8 months/year and intensively raised Assaf dairy flocks housed most time and at higher stocking density in less ventilated buildings than other flocks. Most flocks raised replacement lambs naturally with their dams until weaning and as a separate flock thereafter until lambing at one year of age. Seroprevalence (95% confidence intervals) was 77%, 25% and 5% (4–6) in intensive, semi-intensive and extensive flocks, respectively and the median (interquartile range) flock-seroprevalence was 82% (66–94) in intensive flocks, 31% (14–31) in semi-intensive flocks and 4% (0–7) in extensive flocks. Seroprevalence was lowest in one year-old sheep and increased to flock levels during the year after introduction into the adult flock in most intensive flocks and more gradually in other flocks. Adult flock seroprevalence was associated with housing time but this relationship was not evident within a particular rearing system, indicating that other unknown factors are critical in horizontal MVV-transmission. Low seroprevalence in extensive flocks further supports previous indications that lactogenic MVV-infection is relatively inefficient and horizontal transmission is necessary to ensure long-term maintenance of MVV and this could explain that MVV has not been reported from countries with mainly extensively reared sheep such as Australia and New Zealand. Moreover, it indicates that MVV-control in extensive and semi-intensive flocks can be simple and inexpensive.