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Li, Jun

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https://academica-e.unavarra.es/handle/2454/49404

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Li

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Jun

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Instituto de AgrobiotecnologĆ­a (IdAB)

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Start date: 2011 Ɨ End date: 2016 Ɨ Subject: Carbohydrate metabolism Ɨ Author: Bahaji, Abdellatif Ɨ Author: Almagro Zabalza, Goizeder Ɨ

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    Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production
    (National Academy of Sciences, 2011) Baroja FernĆ”ndez, Edurne; MuƱoz PĆ©rez, Francisco JosĆ©; Li, Jun; Bahaji, Abdellatif; Almagro Zabalza, Goizeder; Montero Macarro, Manuel; Etxeberria, Ed; Hidalgo Cruz, Maite; Sesma Pascual, MarĆ­a Teresa; Pozueta Romero, Javier; IdAB. Instituto de AgrobiotecnologĆ­a / Agrobioteknologiako Institutua; Universidad PĆŗblica de Navarra / Nafarroako Unibertsitate Publikoa
    Sucrose synthase (SUS) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose. In Arabidopsis, a multigene family encodes six SUS (SUS1-6) isoforms. The involvement of SUS in the synthesis of UDP-glucose and ADP-glucose linked to Arabidopsis cellulose and starch biosynthesis, respectively, has been questioned by Barratt et al. [(2009) Proc Natl Acad Sci USA 106:13124ā€“13129], who showed that (i) SUS activity in wild type (WT) leaves is too low to account for normal rate of starch accumulation in Arabidopsis, and (ii) different organs of the sus1/sus2/sus3/sus4 SUS mutant impaired in SUS activity accumulate WT levels of ADP-glucose, UDP-glucose, cellulose and starch. However, these authors assayed SUS activity under unfavorable pH conditions for the reaction. By using favorable pH conditions for assaying SUS activity, in this work we show that SUS activity in the cleavage direction is sufficient to support normal rate of starch accumulation in WT leaves. We also demonstrate that sus1/sus2/sus3/sus4 leaves display WT SUS5 and SUS6 expression levels, whereas leaves of the sus5/sus6 mutant display WT SUS1ā€“4 expression levels. Furthermore, we show that SUS activity in leaves and stems of the sus1/sus2/sus3/sus4 and sus5/sus6 plants is ~85% of that of WT leaves, which can support normal cellulose and starch biosynthesis. The overall data disprove Barratt et al. (2009) claims, and are consistent with the possible involvement of SUS in cellulose and starch biosynthesis in Arabidopsis.