Goicoa Mangado, Tomás
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Goicoa Mangado
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Tomás
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Estadística, Informática y Matemáticas
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InaMat2. Instituto de Investigación en Materiales Avanzados y Matemáticas
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Publication Open Access The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis(Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), 2017) Pereira, Catia; Montalbán, Itziar A.; Goicoa Mangado, Tomás; Ugarte Martínez, María Dolores; Correia, Sandra; Canhoto, Jorge M.; Moncaleán, Paloma; Estadística e Investigación Operativa; Estatistika eta Ikerketa OperatiboaAim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability.Publication Open Access Pinus spp. somatic embryo conversion under high temperature: effect on the morphological and physiological characteristics of plantlets(MDPI, 2020) Marqués do Nascimento, Antonia Maiara; Barroso, Priscila Alves; Ferreira do Nascimento, Naysa Flavia; Goicoa Mangado, Tomás; Ugarte Martínez, María Dolores; Montalbán, Itziar A.; Moncaleán, Paloma; Estatistika, Informatika eta Matematika; Institute for Advanced Materials and Mathematics - INAMAT2; Estadística, Informática y MatemáticasClimatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in Pinus spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60◦C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from Pinus radiata D.Don (Radiata pine) and Pinus halepensis Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of P. radiata submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, P. radiata plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60◦C) had a significant increase in gs and E. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE.