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Pozueta Romero, Javier

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https://academica-e.unavarra.es/handle/2454/49806

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Pozueta Romero

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Javier

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Instituto de AgrobiotecnologĆ­a (IdAB)

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0000-0002-0335-9663

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2094

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2004 - 200912010 - 20181
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Author: Viale Bailone, Alejandro M. Ɨ Subject: ADP-glucose Ɨ

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    PublicationOpen Access
    Most of ADP-glucose linked to starch biosynthesis occurs outside the chloroplast in source leaves
    (National Academy of Sciences, 2004) Baroja FernĆ”ndez, Edurne; MuƱoz PĆ©rez, Francisco JosĆ©; Zandueta Criado, Aitor; MorĆ”n Zorzano, MarĆ­a Teresa; Viale Bailone, Alejandro M.; Alonso CasajĆŗs, Nora; Pozueta Romero, Javier; IdAB. Instituto de AgrobiotecnologĆ­a / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua
    Sucrose and starch are end products of two segregated gluconeogenic pathways, and their production takes place in the cytosol and chloroplast of green leaves, respectively. According to this view, the plastidial ADP glucose (ADPG) pyrophosphorylase (AGP) is the sole enzyme catalyzing the synthesis of the starch precursor molecule ADPG. However, a growing body of evidences indicates that starch formation involves the import of cytosolic ADPG to the chloroplast. This evidence is consistent with the idea that synthesis of the ADPG linked to starch biosynthesis takes place in the cytosol by means of sucrose synthase, whereas AGP channels the glucose units derived from the starch breakdown. To test this hypothesis, we first investigated the subcellular localization of ADPG. Toward this end, we constructed transgenic potato plants that expressed the ADPG-cleaving adenosine diphosphate sugar pyrophosphatase (ASPP) from Escherichia coli either in the chloroplast or in the cytosol. Source leaves from plants expressing ASPP in the chloroplast exhibited reduced starch and normal ADPG content as compared with control plants. Most importantly however, leaves from plants expressing ASPP in the cytosol showed a large reduction of the levels of both ADPG and starch, whereas hexose phosphates increased as compared with control plants. No pleiotropic changes in photosynthetic parameters and maximum catalytic activities of enzymes closely linked to starch and sucrose metabolism could be detected in the leaves expressing ASPP in the cytosol. The overall results show that, essentially similar to cereal endosperms, most of the ADPG linked to starch biosynthesis in source leaves occurs in the cytosol.
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    PublicationOpen Access
    A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters
    (Nature Research, 2018) Almagro Zabalza, Goizeder; Viale Bailone, Alejandro M.; Montero Macarro, Manuel; MuƱoz PĆ©rez, Francisco JosĆ©; Baroja FernĆ”ndez, Edurne; Mori, Hirotada; Pozueta Romero, Javier; IdAB. Instituto de AgrobiotecnologĆ­a / Agrobioteknologiako Institutua; Universidad PĆŗblica de Navarra / Nafarroako Unibertsitate Publikoa
    ADP-glucose is the precursor of glycogen biosynthesis in bacteria, and a compound abundant in the starchy plant organs ingested by many mammals. Here we show that the enteric species Escherichia coli is capable of scavenging exogenous ADP-glucose for use as a glycosyl donor in glycogen biosynthesis and feed the adenine nucleotide pool. To unravel the molecular mechanisms involved in this process, we screened the E. coli single-gene deletion mutants of the Keio collection for glycogen content in ADP-glucose-containing culture medium. In comparison to wild-type (WT) cells, individual āˆ†nupC and āˆ†nupG mutants lacking the cAMP/CRP responsive inner-membrane nucleoside transporters NupC and NupG displayed reduced glycogen contents and slow ADP-glucose incorporation. In concordance, āˆ†cya and āˆ†crp mutants accumulated low levels of glycogen and slowly incorporated ADP-glucose. Two-thirds of the glycogen-excess mutants identified during screening lacked functions that underlie envelope biogenesis and integrity, including the RpoE specific RseA anti-sigma factor. These mutants exhibited higher ADP-glucose uptake than WT cells. The incorporation of either āˆ†crp, āˆ†nupG or āˆ†nupC null alleles sharply reduced the ADP-glucose incorporation and glycogen content initially witnessed in āˆ†rseA cells. Overall, the data showed that E. coli incorporates extracellular ADP-glucose through a cAMP/CRP-regulated process involving the NupC and NupG nucleoside transporters that is facilitated under envelope stress conditions.