Person:
Arrese-Igor Sánchez, César

person.page.identifierURI

https://academica-e.unavarra.es/handle/2454/48608

Last Name

Arrese-Igor Sánchez

First Name

César

person.page.departamento

Ciencias

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IMAB. Research Institute for Multidisciplinary Applied Biology

ORCID

0000-0002-2195-4458

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48

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Now showing 1 - 10 of 14

Open Access
Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean
(Oxford University Press, 2013) Gil Quintana, Erena; Larrainzar Rodríguez, Estíbaliz; Seminario Huárriz, Amaia; Díaz Leal, Juan Luis; Alamillo, Josefa M.; Pineda, Manuel; Arrese-Igor Sánchez, César; Wienkoop, Stefanie; González García, Esther; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa: 735/2008; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa: 134/2012
Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in the water-deprived root system while nitrogenase activity was maintained at control values in the well-watered half. Concomitantly, amino acids and ureides accumulated in the water-deprived belowground organs regardless of transpiration rates. Ureide accumulation was found to be related to the decline in their degradation activities rather than increased biosynthesis. Finally, proteomic analysis suggests that plant carbon metabolism, protein synthesis, amino acid metabolism, and cell growth are among the processes most altered in soybean nodules under drought stress. Results presented here support the hypothesis of a local regulation of NF taking place in soybean and downplay the role of ureides in the inhibition of NF
Open Access
Is N-feedback involved in the inhibition of nitrogen fixation in drought-stressed Medicago truncatula?
(Oxford University Press, 2013) Gil Quintana, Erena; Larrainzar Rodríguez, Estíbaliz; Arrese-Igor Sánchez, César; González García, Esther; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa: 735/2008
Drought stress is a major factor limiting nitrogen fixation (NF) in crop production. However, the regulatory mechanism involved and the origin of the inhibition, whether local or systemic, is still controversial and so far scarcely studied in temperate forage legumes. Medicago truncatula plants were symbiotically grown with a split-root system and exposed to gradual water deprivation. Physiological parameters, NF activity, and amino acid content were measured. The partial drought treatment inhibited NF in the nodules directly exposed to drought stress. Concomitantly, in the droughted below-ground organs, amino acids accumulated prior to any drop in evapotranspiration (ET). It is concluded that drought exerts a local inhibition of NF and drives an overall accumulation of amino acids in diverse plant organs which is independent of the decrease in ET. The general increase in the majority of single amino acids in the whole plant questions the commonly accepted concept of a single amino acid acting as an N-feedback signal.
Open Access
Split‐root systems applied to the study of the legume‐rhizobial symbiosis: what have we learned?
(Wiley, 2014) Larrainzar Rodríguez, Estíbaliz; Gil Quintana, Erena; Arrese-Igor Sánchez, César; González García, Esther; Marino Bilbao, Daniel; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
Split-root system (SRS) approaches allow the differential treatment of separate and independent root systems, while sharing a common aerial part. As such, SRS is a useful tool for the discrimination of systemic (shoot origin) versus local (root/nodule origin) regulation mechanisms. This type of approach is particularly useful when studying the complex regulatory mechanisms governing the symbiosis established between legumes and Rhizobium bacteria. The current work provides an overview of the main insights gained from the application of SRS approaches to understand how nodule number (nodulation autoregulation) and nitrogen fixation are controlled both under non-stressful conditions and in response to a variety of stresses. Nodule number appears to be mainly controlled at the systemic level through a signal which is produced by nodule/root tissue, translocated to the shoot, and transmitted back to the root system, involving shoot Leu-rich repeat receptor-like kinases. In contrast, both local and systemic mechanisms have been shown to operate for the regulation of nitrogenase activity in nodules. Under drought and heavy metal stress, the regulation is mostly local, whereas the application of exogenous nitrogen seems to exert a regulation of nitrogen fixation both at the local and systemic levels.
Open Access
Physiological responses of legume nodules to drought
(Global Science Books, 2011) Arrese-Igor Sánchez, César; González García, Esther; Marino Bilbao, Daniel; Ladrera Fernández, Rubén; Larrainzar Rodríguez, Estíbaliz; Gil Quintana, Erena; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
Legumes include important agricultural crops, as their high protein content is of primary importance for human food and animal feed. In addition, the ability of most of them to establish symbiotic relationships with soil bacteria allows them to obtain their N requirements from nitrogen fixation in nodules and, therefore, avoids the use of nitrogen fertilizers. Thus, legumes are also essential to improve the soil fertility and quality of agricultural lands and to reclaim eroded or barren areas, making them crucial for agricultural and environmental sustainability. However, legume nitrogen fixation in crop species is very sensitive to environmental constraints and drought, in particular. The present contribution reviews our current knowledge on the processes involved in this inhibition, with particular emphasis on oxygen, nitrogen and carbon physiology. Emerging aspects such as oxidative damage, C/N interactions and sulphur metabolism together with future prospects are also discussed.
Open Access
Long-term mannitol-induced osmotic stress leads to stomatal closure, carbohydrate accumulation and changes in leaf elasticity in Phaselous vulgaris leaves
(Academic Journals, 2010) Sassi, Sameh; Aydi, Samir; Hessini, Kamel; González García, Esther; Arrese-Igor Sánchez, César; Abdelly, Chedly; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
The effect of long-term osmotic stress was investigated in leaves of two common bean lines, with contrasting tolerance: Flamingo (tolerant) and coco blanc (sensitive). Water relations, organic solute, ion accumulation and amino acids content as well as osmotic adjustment (OA) were studied during an extended exposure to osmotic stress. Osmotic stress was applied by means of 50 mM mannitol for 15 days. At the end of the stress period, both osmotic potential at full turgor (psi(100)) and at turgor loss point (psi(0)) decreased significantly in stressed plants compared with the control. The decrease being greater in the sensitive line, showed a greater OA compared with flamingo. Sugars contents increased in stressed plants and seem to be the major components of osmotic adjustment in stressed common bean leaves. The increase was more marked in coco blanc. Osmotic stress tolerance could thus not be associated with higher OA. The possible role of decreased leaf cell elasticity (epsilon(max)) is discussed in relation to osmotic stress tolerance in this species.
Open Access
Nodule performance within a changing environmental context
(Elsevier, 2014) Aranjuelo Michelena, Iker; Arrese-Igor Sánchez, César; Molero, Gemma; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
Global climate models predict that future environmental conditions will see alterations in temperature, water availability and CO2 concentration ([CO2]) in the atmosphere. Climate change will reinforce the need to develop highly productive crops. For this purpose it is essential to identify target traits conditioning plant performance in changing environments. N2 fixing plants represent the second major crop of agricultural importance worldwide. The current review provides a compilation of results from existing literature on the effects of several abiotic stress conditions on nodule performance and N2 fixation. The environmental factors analysed include water stress, salinity, temperature, and elevated [CO2]. Despite the large number of studies analysing [CO2] effects in plants, frequently they have been conducted under optimal growth conditions that are difficult to find in natural conditions where different stresses often occur simultaneously. This is why we have also included a section describing the current state of knowledge of interacting environmental conditions in nodule functioning. Regardless of the environmental factor considered, it is evident that some general patterns of nodule response are observed. Nodule carbohydrate and N compound availability, together with the presence of oxygen reactive species (ROS) have proven to be the key factors modulating N2 fixation at the physiological/biochemical levels. However, with the exception of water availability and [CO2], it should also be considered that nodule performance has not been characterised in detail under other limiting growth conditions. This highlights the necessity to conduct further studies considering these factors. Finally, we also observe that a better understanding of these metabolic effects of changing environment in nodule functioning would require an integrated and synergistic investigation based on widely used and novel protocols such as transcriptomics, proteomics, metabolomics and stable isotopes.
Open Access
Nodule carbohydrate catabolism is enhanced in the Medicago truncatula A17-Sinorhizobium medicae WSM419 symbiosis
(Frontiers Media, 2014) Larrainzar Rodríguez, Estíbaliz; Gil Quintana, Erena; Seminario Huárriz, Amaia; Arrese-Igor Sánchez, César; González García, Esther; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
The symbiotic association between Medicago truncatula and Sinorhizobium meliloti is a well-established model system in the legume–Rhizobium community. Despite its wide use, the symbiotic efficiency of this model has been recently questioned and an alternative microsymbiont, S. medicae, has been proposed. However, little is known about the physiological mechanisms behind the higher symbiotic efficiency of S. medicae WSM419. In the present study, we inoculated M. truncatula Jemalong A17 with either S. medicae WSM419 or S. meliloti 2011 and compared plant growth, photosynthesis, N2-fixation rates, and plant nodule carbon and nitrogen metabolic activities in the two systems. M. truncatula plants in symbiosis with S. medicae showed increased biomass and photosynthesis rates per plant. Plants grown in symbiosis with S. medicae WSM419 also showed higher N2-fixation rates, which were correlated with a larger nodule biomass, while nodule number was similar in both systems. In terms of plant nodule metabolism, M. truncatula–S. medicae WSM419 nodules showed increased sucrose-catabolic activity, mostly associated with sucrose synthase, accompanied by a reduced starch content, whereas nitrogen-assimilation activities were comparable to those measured in nodules infected with S. meliloti 2011. Taken together, these results suggest that S. medicae WSM419 is able to enhance plant carbon catabolism in M. truncatula nodules, which allows for the maintaining of high symbiotic N2-fixation rates, better growth and improved general plant performance.
Open Access
Physiological, hormonal and metabolic responses of two alfalfa cultivars with contrasting responses to drought
(MDPI, 2019) Soba Hidalgo, David; Zhou, Bangwei; Arrese-Igor Sánchez, César; Munné Bosch, Sergi; Aranjuelo Michelena, Iker; Zientziak; Institute for Multidisciplinary Research in Applied Biology - IMAB; Ciencias; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Alfalfa (Medicago sativa L.) is frequently constrained by environmental conditions such as drought. Within this context, it is crucial to identify the physiological and metabolic traits conferring a better performance under stressful conditions. In the current study, two alfalfa cultivars (San Isidro and Zhong Mu) with different physiological strategies were selected and subjected to water limitation conditions. Together with the physiological analyses, we proceeded to characterize the isotopic, hormone, and metabolic profiles of the different plants. According to physiological and isotopic data, Zhong Mu has a water-saver strategy, reducing water lost by closing its stomata but fixing less carbon by photosynthesis, and therefore limiting its growth under water-stressed conditions. In contrast, San Isidro has enhanced root growth to replace the water lost through transpiration due to its more open stomata, thus maintaining its biomass. Zhong Mu nodules were less able to maintain nodule N2 fixing activity (matching plant nitrogen (N) demand). Our data suggest that this cultivar-specific performance is linked to Asn accumulation and its consequent N-feedback nitrogenase inhibition. Additionally, we observed a hormonal reorchestration in both cultivars under drought. Therefore, our results showed an intra-specific response to drought at physiological and metabolic levels in the two alfalfa cultivars studied.
Open Access
A proteomic approach reveals new actors of nodule response to drought in split-root grown pea plants
(Wiley, 2014) Irar, Sami; González García, Esther; Arrese-Igor Sánchez, César; Marino Bilbao, Daniel; Ciencias del Medio Natural; Natura Ingurunearen Zientziak
Drought is considered the more harmful abiotic stress resulting in crops yield loss. Legumes in symbiosis with rhizobia are able to fix atmospheric nitrogen. Biological nitrogen fixation (SNF) is a very sensitive process to drought and limits legumes agricultural productivity. Several factors are known to regulate SNF including oxygen availability to bacteroids, carbon and nitrogen metabolisms; but the signalling pathways leading to SNF inhibition are largely unknown. In this work, we have performed a proteomic approach of pea plants grown in split-root-system where one half of the root was well-irrigated and the other was subjected to drought. Water stress locally provoked nodule water potential decrease that led to SNF local inhibition. The proteomic approach revealed 11 and 7 nodule proteins regulated by drought encoded by P. sativum and R. leguminosarum genomes respectively. Among these 18 proteins, three proteins related to flavonoid metabolism, two to sulphur metabolism and three RNA-binding proteins were identified. These proteins could be molecular targets for future studies focused on the improvement of legumes tolerance to drought. Moreover, this work also provides new hints for the deciphering of SNF regulation machinery in nodules.
Open Access
Application of anti-transpirants temporarily alleviates the inhibition of symbiotic nitrogen fixation in drought-stressed pea plants
(Elsevier, 2019) Aldasoro Galán, Joseba; Larrainzar Rodríguez, Estíbaliz; Arrese-Igor Sánchez, César; Ciencias; Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Stomatal closure is one of the first plant responses under a water deficit situation. This leads to a decline in transpiration but also in the plant photosynthetic activity. Legume plants grown under symbiosis with rhizobium bacteria present an inhibition of nitrogen fixation that has been shown to occur even before this of photosynthesis. One of the hypotheses to explain this rapid inhibition is the accumulation of nitrogen (N) compounds in nodules due to reduced transpiration, which would provoke the N-feedback inhibition of nitrogenase activity. The current work analyzes the effects of changes in transpiration rates in the regulation of nitrogen fixation through the application of the anti-transpirant Vapor Gard (VG) to pea (Pisum sativum L.) plants subjected to a progressive water deficit. VG produced a rapid inhibition of nitrogen fixation upon application. This inhibition, however, did not coincide with the accumulation of either amino acids or soluble carbohydrates observed at later drought stages in nodules. Results show that the application of VG has a beneficial, albeit temporary, effect in both maintaining the plant water status and apparent nitrogenase activity of nodulated pea plants under water-deficit conditions.