Dorado Morales, Pedro

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https://academica-e.unavarra.es/handle/2454/48915

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Dorado Morales

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Pedro

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Producción Agraria

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0000-0001-5760-1999

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750816

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811141

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2020 - 20212
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Subject: Rhodococcus erythropolis ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Elevated c-di-GMP levels promote biofilm formation and biodesulfurization capacity of Rhodococcus erythropolis
    (Society for Applied Microbiology, 2020) Dorado Morales, Pedro; Martínez, Igor; Rivero Buceta, Virginia; Díaz, Eduardo; Bähre, Heike; Lasa Uzcudun, Íñigo; Solano Goñi, Cristina; Ciencias de la Salud; Osasun Zientziak
    Bacterial biofilms provide high cell density and a superior adaptation and protection from stress conditions compared to planktonic cultures, making them a very promising approach for bioremediation. Several Rhodococcus strains can desulfurize dibenzothiophene (DBT), a major sulphur pollutant in fuels, reducing air pollution from fuel combustion. Despite multiple efforts to increase Rhodococcus biodesulfurization activity, there is still an urgent need to develop better biocatalysts. Here, we implemented a new approach that consisted in promoting Rhodococcus erythropolis biofilm formation through the heterologous expression of a diguanylate cyclase that led to the synthesis of the biofilm trigger molecule cyclic di-GMP (c-di-GMP). R. erythropolis biofilm cells displayed a significantly increased DBT desulfurization activity when compared to their planktonic counterparts. The improved biocatalyst formed a biofilm both under batch and continuous flow conditions which turns it into a promising candidate for the development of an efficient bioreactor for the removal of sulphur heterocycles present in fossil fuels.
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    PublicationOpen Access
    Genetic tools derived from Staphylococcus aureus for biotechnological applications in Gram-positive bacteria
    (2021) Dorado Morales, Pedro; Lasa Uzcudun, Íñigo; Solano Goñi, Cristina; Ciencias de la Salud; Osasun Zientziak
    Staphylococcus aureus is a versatile human pathogen that has emerged as one of the most successful infectious agents of recent times, able to cause a range of diseases including skin and soft tissue infections, endocarditis, sepsis, pneumonia, osteomyelitis, bacteremia, and abscesses in organ tissues. Besides its clinical relevance, S. aureus has served as a model to study fundamental cellular processes, such as biofilm formation, the regulatory functions of small RNAs or growth and division of spherical cocci. Based on the accumulated knowledge of S. aureus biology, the availability of database resources and the advances in high-throughput genome sequencing, in this work we have aimed at developing new genetic tools derived from S. aureus for biotechnological applications in Gram-positive bacteria.