(Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006) Castellón Gadea, Jordi; Pisabarro de Lucas, Gerardo; Ramírez Nasto, Lucía; Producción Agraria; Nekazaritza Ekoizpena
Pleurotus ostreatus is a commercially important edible mushroom commonly
known as oyster mushroom which has also important biotechnical applications.
Industrial production of P.ostreatus is based on a solid fermentation
process in which a limited number of selected strains are used. Optimization
of industrial mushroom production depends on improving the culture
process and breeding new strains with higher yields and productivities. In a
previous study a linkage map of P. ostreatus strain N001 was constructed,
which provided a basis for performing an efficient QTL (Quantitative trait
loci) analysis based in a population of 80 sibling monokaryons. The map is
based on the segregation of RAPD markers, RFLP markers, phenotypic
characters and cloned genes. Nevertheless the linkage map is just a first step
towards the selection of the appropiate parentals for new breeds.
In order to organize and improve the access to the data and information
accumulated in the previous works mentioned above, a Microsoft® Excel
Linkage Map Matrix (MELMM) was designed and created. On this linkage
map matrix we could have an easy and functional view of the P. ostreatus linkage
map data, such as, recombination frequencies, genotypes information
and degree of similarity between monokaryons that will help us in the design
of breeding crosses aimed at improving QTLs of agronomic interest of new
commercial strains.
