(Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006) Palma Dovis, Leopoldo; Peñas Parrila, María Manuela; Ramírez Nasto, Lucía; Pisabarro de Lucas, Gerardo; Producción Agraria; Nekazaritza Ekoizpena
Pleurotus ostreatus, commonly known as oyster mushroom, is a commercially
important edible fungus with interesting biotechnological properties. Quantitative
trait loci (QTL) analyses are rare in fungi and little is known about
their number, position, and genetic structure. Previous studies of our group
have allowed the construction of a genetic linkage map of P. ostreatus var.
florida, which has provided the basis for performing an efficient QTL analysis.
In fact, there is a region of the chromosome VII of P. ostreatus where the
most QTLs related to the production and precocity characters have been
mapped. These quantitative traits are presumably under the control of a
polygenic genetic system and could be associated with some chromosomal
regions. The hypothesis of this work is that there is a region in the chromosome
VII of protoclon PC15 (monokaryotic parental of the N001 dikaryotic
strain) where exist genes which are responsible for the QTLs mentioned
above. In order to test this hypothesis, we are developing a molecular QTL
analysis through the sequencing of a region with an approximated size of 320
Kbp in chromosome VII (protoclon PC15). For this purpose, a BAC genomic
library was constructed and two BAC clones spanning the region of
interest are being sequenced. To carry out an efficient computational prediction
of protein-coding genes and its annotation on the partial sequences obtained
up to date, we have used different Internet resources such as
BLASTx, BLASTp, BLASTn, and FGENESH trained on some basidiomycetes
genomic data like Phanerochaete chrysosporium and Cryptococcus
neoformans (SoftBerry). To our knowledge, this is the firs molecular QTL
analysis performed on this edible mushroom.
