Gil Monreal, Miriam
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Gil Monreal
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Miriam
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Publication Open Access Induction of the PDH bypass and upregulation of the ALDH7B4 in plants treated with herbicides inhibiting amino acid biosynthesis(Elsevier, 2017) Gil Monreal, Miriam; Zabalza Aznárez, Ana; Missihoun, Tagnon D.; Dormann, Peter; Bartels, Dorothea; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaImazamox and glyphosate represent two classes of herbicides that inhibit the activity of acetohydroxyacid synthase in the branched-chain amino acid biosynthesis pathway and the activity of 5-enolpyruvylshikimate-3-phosphate synthase in the aromatic amino acid biosynthesis pathway, respectively. However, it is still unclear how imazamox and glyphosate lead to plant death. Both herbicides inhibit amino-acid biosynthesis and were found to induce ethanol fermentation in plants, but an Arabidopsis mutant deficient in alcohol dehydrogenase 1 was neither more susceptible nor more resistant than the wild-type to the herbicides. In this study, we investigated the effects of the amino acid biosynthesis inhibitors, imazamox and glyphosate, on the pyruvate dehydrogenase bypass reaction and fatty acid metabolism in A. thaliana. We found that the pyruvate dehydrogenase bypass was upregulated following the treatment by the two herbicides. Our results suggest that the Arabidopsis aldehyde dehydrogenase 7B4 gene might be participating in the pyruvate dehydrogenase bypass reaction. We evaluated the potential role of the aldehyde dehydrogenase 7B4 upon herbicide treatment in the plant defence mechanism. Plants that overexpressed the ALDH7B4 gene accumulated less soluble sugars, starch, and fatty acids and grew better than the wild-type after herbicide treatment. We discuss how the upregulation of the ALDH7B4 alleviates the effects of the herbicides, potentially through the detoxification of the metabolites produced in the pyruvate dehydrogenase bypass.Publication Open Access La sobreexpresión del gen ALDH7B4 alivia los efectos provocados por herbicidas inhibidores de la biosíntesis de aminoácidos(Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2017) Gil Monreal, Miriam; Zabalza Aznárez, Ana; Missihoun, Tagnon D.; Bartels, Dorothea; Royuela Hernando, Mercedes; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaEn este trabajo se ha profundizado en el conocimiento de los efectos fisiológicos provocados por los herbicidas imazamox y glifosato. En concreto, se ha estudiado el papel de la ALDH7B4, aldehído deshidrogenasa, que contribuye a la tolerancia de las plantas a diversos estreses. Se cultivaron plantas de Arabidopsis thaliana silvestres y plantas que sobreexpresan el gen ALDH7B4, que fueron tratadas con dosis comparables de imazamox o glifosato. Se observó que ambos genotipos presentan valores similares de malondialdehído, indicando que la ALDH7B4 no participa en la detoxificación de aldehídos derivados de la peroxidación lipídica. Por otro lado, se monitorizaron los principales efectos fisiológicos provocados por este tipo de herbicidas y se observó que los efectos de los herbicidas (contenido en carbohidratos y crecimiento) se atenúan en las plantas mutantes, indicando que la ALDH7B4 contribuye a aliviar los efectos provocados por estos herbicidas.