Glaría Ezquer, Idoia

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https://academica-e.unavarra.es/handle/2454/49149

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Glaría Ezquer

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Idoia

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Producción Agraria

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0000-0003-4556-489X

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6796

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2011 - 20132
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Author: Amorena Zabalza, Beatriz × Subject: Dendritic cell ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection
    (BioMed Central, 2011) Crespo Otano, Helena; Reina Arias, Ramsés; Glaría Ezquer, Idoia; Ramírez Álvarez, Hugo; Andrés, Ximena de; Jauregui, Paula; Luján, Lluís; Martínez Pomares, Luisa; Amorena Zabalza, Beatriz; Andrés Cara, Damián de; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    This study aims to characterize the mannose receptor (MR) gene in sheep and its role in ovine visna/maedi virus (VMV) infection. The deduced amino acid sequence of ovine MR was compatible with a transmembrane protein having a cysteine-rich ricin-type amino-terminal region, a fibronectin type II repeat, eight tandem C-type lectin carbohydrate-recognition domains (CRD), a transmembrane region, and a cytoplasmic carboxy-terminal tail. The ovine and bovine MR sequences were closer to each other compared to human or swine MR. Concanavalin A (ConA) inhibited VMV productive infection, which was restored by mannan totally in ovine skin fibroblasts (OSF) and partially in blood monocyte-derived macrophages (BMDM), suggesting the involvement of mannosylated residues of the VMV ENV protein in the process. ConA impaired also syncytium formation in OSF transfected with an ENV-encoding pN3-plasmid. MR transcripts were found in two common SRLV targets, BMDM and synovial membrane (GSM) cells, but not in OSF. Viral infection of BMDM and especially GSM cells was inhibited by mannan, strongly suggesting that in these cells the MR is an important route of infection involving VMV Env mannosylated residues. Thus, at least three patterns of viral entry into SRLV-target cells can be proposed, involving mainly MR in GSM cells (target in SRLV-induced arthritis), MR in addition to an alternative route in BMDM (target in SRLV infections), and an alternative route excluding MR in OSF (target in cell culture). Different routes of SRLV infection may thus coexist related to the involvement of MR differential expression.
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    PublicationOpen Access
    Small ruminant macrophage polarization may play a pivotal role on lentiviral infection
    (BioMed Central, 2013) Crespo Otano, Helena; Bertolotti, Luigi; Juganaru, Magda; Glaría Ezquer, Idoia; Andrés Cara, Damián de; Amorena Zabalza, Beatriz; Rosati, Sergio; Reina Arias, Ramsés; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua: IIQ14064.RI1; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Small ruminant lentiviruses (SRLV) infect the monocyte/macrophage lineage inducing a long-lasting infection affecting body condition, production and welfare of sheep and goats all over the world. Macrophages play a pivotal role on the host's innate and adaptative immune responses against parasites by becoming differentially activated. Macrophage heterogeneity can tentatively be classified into classically differentiated macrophages (M1) through stimulation with IFN-gamma displaying an inflammatory profile, or can be alternatively differentiated by stimulation with IL-4/IL-13 into M2 macrophages with homeostatic functions. Since infection by SRLV can modulate macrophage functions we explored here whether ovine and caprine macrophages can be segregated into M1 and M2 populations and whether this differential polarization represents differential susceptibility to SRLV infection. We found that like in human and mouse systems, ovine and caprine macrophages can be differentiated with particular stimuli into M1/M2 subpopulations displaying specific markers. In addition, small ruminant macrophages are plastic since M1 differentiated macrophages can express M2 markers when the stimulus changes from IFN-gamma to IL-4. SRLV replication was restricted in M1 macrophages and increased in M2 differentiated macrophages respectively according to viral production. Identification of the infection pathways in macrophage populations may provide new targets for eliciting appropriate immune responses against SRLV infection.