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Pérez Prados, Antonio

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https://academica-e.unavarra.es/handle/2454/49783

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Pérez Prados

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Antonio

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Estadística, Informática y Matemáticas

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0000-0002-6496-4242

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34

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20132
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Subject: Human papillomavirus (HPV) × Subject: Urine samples ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
    (American Society for Microbiology, 2013) Muñoz Bobo, Marina; Camargo, Milena; Soto de León, Sara; Sánchez, Ricardo; Pineda Peña, Andrea; Pérez Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso; Estadística e Investigación Operativa; Estatistika eta Ikerketa Operatiboa
    Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.
  • Thumbnail Image
    PublicationOpen Access
    Human papillomavirus detection from human immunodeficiency virus-infected Colombian women’s paired urine and cervical samples
    (Public Library of Science, 2013) Muñoz Bobo, Marina; Camargo, Milena; Soto de León, Sara; Sánchez, Ricardo; Parra, Diana; Pineda Peña, Andrea; Sussmann, Otto; Pérez Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso; Estadística e Investigación Operativa; Estatistika eta Ikerketa Operatiboa
    Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.