López Maestresalas, Ainara

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López Maestresalas

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Ainara

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Ingeniería

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IS-FOOD. Research Institute on Innovation & Sustainable Development in Food Chain

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0000-0001-6636-0752

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750902

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810716

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Now showing 1 - 6 of 6

Open Access
On-site identification of esca-affected vines using hyperspectral imaging
(Hellenic Society of Agricultural Engineers, 2025) León Ecay, Sara; Ruiz de Gauna González, Jon; López Maestresalas, Ainara; Jarén Ceballos, Carmen; Arazuri Garín, Silvia; Ingeniería; Ingeniaritza; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
Esca represents one of the greatest threats to modern viticulture as it causes large annual economic losses. At present, there is a lack of effective strategies for disease control, so a technique capable of detecting affected vines would allow annual monitoring of disease incidence in the vineyard leading to a better crop management and decision making. This study evaluates close-range hyperspectral imaging for the detection of esca naturally infected vines. Images of 11 vines of the Tempranillo variety grown on plots in Bodegas Otazu, in Etxauri (Navarre, Spain) were acquired. A Specim IQ snapshot hyperspectral camera was used to record the images on August, 21 2023 on the field under natural light conditions. The camera has a spectral resolution of 7 nm (204 wavelengths) and a spatial resolution of 512 x 512 in the 400 ¿ 1000 nm spectral range (Vis-NIR). An individual image was acquired for each vine, of which 9 were symptomatic and 2 asymptomatic. Three classes were analysed: asymptomatic leaves of asymptomatic vines (Class 1), asymptomatic leaves of symptomatic vines (Class 2) and asymptomatic areas of symptomatic leaves of symptomatic vines (Class 3). A total of 300 pixels were randomly selected, 100 per class, for further analysis. Partial Least Square Discriminant Analysis (PLSDA) was used to classify the pixels into the three categories. An accuracy of 86% was achieved in the cross-validation dataset. Models were externally validated using an image of an asymptomatic vine and an image of a symptomatic vine. The visualisation of the images showed that the majority of the pixels of the asymptomatic vine image were classified as class 1, while most of the pixels of the symptomatic vine image were classified as either class 2 or class 3. Hence, this study demonstrated the potential of close-range HSI for the on-site detection of esca.
Open Access
Early detection of Esca disease in grapevines using in-field hyperspectral proximal sensing
(Hellenic Society of Agricultural Engineers, 2025) López Maestresalas, Ainara; Ruiz de Gauna González, Jon; Jarén Ceballos, Carmen; León Ecay, Sara; Arazuri Garín, Silvia; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ingeniería; Ingeniaritza; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
Esca is one of the most destructive vine diseases in the world. It causes significant economic losses, mainly due to reduced grape yield and quality. Currently, the approved methods of controlling esca include preventive methods such as the use of fungicides on plant wounds or the use of planting systems that do not require intensive pruning, among others. It is therefore advisable to monitor the crop to identify those vines that are susceptible to the disease. For this reason, in this study a proximal hyperspectral camera was used for early detection of esca presence in asymptomatic grapevine leaves. Images of 11 vines of the Tempranillo variety grown in Etxauri (Navarre, Spain) were analysed. Hyperspectral images were acquired using a Specim IQ snapshot camera, mounted on a tripod, working in the range of 400¿1000 nm with a spectral resolution of 7 nm (204 bands), and an image resolution of 512 × 512 pixel including an RGB camera (5 Mpix). The images were taken under natural ambient light conditions on August 21, 2023. From the 11 vines selected, 9 showed visual symptoms of esca and the remaining 2 were asymptomatic to the naked eye. A total of 200 pixels were randomly selected from the dataset, 100 from asymptomatic leaves of asymptomatic vines (class 1) and 100 from asymptomatic leaves of symptomatic vines (class 2). Partial Least Square Discriminant Analysis (PLS-DA) was performed to classify the leaves into the two classes. Classification rates of 97% were achieved in the cross-validation dataset. Models were externally validated at pixel-level using one image of an asymptomatic vine and another of a symptomatic vine. The visualisation of the images confirmed the correct classification of the pixels into the two classes, indicating that by using proximal hyperspectral sensing an early identification of the disease is possible.
Open Access
Potential of NIRS technology for the determination of cannabinoid content in industrial hemp (Cannabis sativa L.)
(MDPI, 2022) Jarén Ceballos, Carmen; Zambrana, P.; Pérez Roncal, Claudia; López Maestresalas, Ainara; Ábrego Arlegui, Andrés; Arazuri Garín, Silvia; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
Industrial hemp (Cannabis sativa L.) is a plant native to Asia, and is considered to be a primary source of food, textile fiber, and medicines. It is characterized by containing minimal concentrations of delta-9 tetrahydrocannabidol (THC), which is the main psychoactive chemical component, and cannabidiol (CBD), a non-psychoactive substance. In most European countries, the maximum concentration legally allowed for cultivation is 0.2% of THC, and it is currently under debate whether to increase this level to 0.3%. Moreover, in many countries its production is being regularized and legalized, increasing the need for a rapid analysis method. The present work evaluated the cannabinoid content in hemp (Cannabis sativa L.) using near infrared spectroscopy (NIRS) technology in combination with chemometric techniques. For this, several samples of the Kompolti variety were analyzed. Samples were dried and ground, and the content of total THC (%) and total CBD (%) was determined by high performance liquid chromatography (HPLC) with a diode array detector as reference measurements, and then the spectra were collected by NIRS. Principal component analysis and partial least square regression models were developed. Good coefficients of determination of cross-validation of 0.77 for THC and CBD, and a ratio of prediction to deviation >2 for total THC and CBD, were achieved. The results obtained show that NIRS technology has potential for the quantitative determination of cannabinoids. Therefore, this analytical method would allow a simpler, more robust, precise, and sustainable estimation than the current HPLC approach.
Open Access
A review of the application of near-infrared spectroscopy for the analysis of potatoes
(American Chemical Society, 2013) López Maestresalas, Ainara; Arazuri Garín, Silvia; García Ruiz, Ignacio; Mangado Ederra, Jesús; Jarén Ceballos, Carmen; Proyectos e Ingeniería Rural; Landa Ingeniaritza eta Proiektuak; Gobierno de Navarra / Nafarroako Gobernua
Potato (Solanum tuberosum L.) is one of the most important crops in the world being considered as a staple food in many developing countries. The potato industry like other vegetable and fruit industries is subject to the current demand of quality products. In order to meet this challenge, the food industry is relying on the adoption of nondestructive and environmentally friendly techniques to determine quality of products. Near-infrared spectroscopy (NIRS) is currently one of the most advanced nondestructive technologies regarding instrumentation and application, and it also complies with the environment requirements as it does not generate emissions or waste. This paper reviews research progress on the analysis of potatoes by NIRS both in terms of determination of constituents and classification according to the different constituents of the tubers. A brief description of the fundamentals of NIRS technology and its advantages over other quality assessment techniques is included. Finally, future prospects of the development of NIRS technology at the industrial level are explored.
Open Access
Classification of beef longissimus thoracis muscle tenderness using hyperspectral imaging and chemometrics
(MDPI, 2022) León Ecay, Sara; López Maestresalas, Ainara; Murillo Arbizu, María Teresa; Beriain Apesteguía, María José; Mendizábal Aizpuru, José Antonio; Arazuri Garín, Silvia; Jarén Ceballos, Carmen; Bass, Phillip D.; Colle, Michael J.; García, David; Romano Moreno, Miguel; Insausti Barrenetxea, Kizkitza; Agronomia, Bioteknologia eta Elikadura; Ingeniaritza; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Agronomía, Biotecnología y Alimentación; Ingeniería; Gobierno de Navarra / Nafarroako Gobernua Universidad Pública de Navarra / Nafarroako Unibertsitate
Nowadays, the meat industry requires non-destructive, sustainable, and rapid methods that can provide objective and accurate quality assessment with little human intervention. Therefore, the present research aimed to create a model that can classify beef samples from longissimus thoracis muscle according to their tenderness degree based on hyperspectral imaging (HSI). In order to obtain different textures, two main strategies were used: (a) aging type (wet and dry aging with or without starters) and (b) aging times (0, 7, 13, 21, and 27 days). Categorization into two groups was carried out for further chemometric analysis, encompassing group 1 (ngroup1 = 30) with samples with WBSF < 53 N whereas group 2 (ngroup2 = 28) comprised samples with WBSF values 53 N. Then, classification models were created by applying the partial least squares discriminant analysis (PLS-DA) method. The best results were achieved by combining the following pre-processing algorithms: 1st derivative + mean center, reaching 70.83% of correctly classified (CC) samples and 67.14% for cross validation (CV) and prediction, respectively. In general, it can be concluded that HSI technology combined with chemometrics has the potential to differentiate and classify meat samples according to their textural characteristics.
Open Access
Hyperspectral imaging to assess the presence of powdery mildew (Erysiphe necator) in cv. Carignan Noir grapevine bunches
(MDPI, 2020) Pérez Roncal, Claudia; López Maestresalas, Ainara; López Molina, Carlos; Jarén Ceballos, Carmen; Urrestarazu Vidart, Jorge; Santesteban García, Gonzaga; Arazuri Garín, Silvia; Ingeniería; Estadística, Informática y Matemáticas; Agronomía, Biotecnología y Alimentación; Ingeniaritza; Estatistika, Informatika eta Matematika; Agronomia, Bioteknologia eta Elikadura; Gobierno de Navarra / Nafarroako Gobernua, Proyecto DECIVID (Res.104E/2017); Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, FPI-UPNA-2017 (Res.654/2017)
Powdery mildew is a worldwide major fungal disease for grapevine, which adversely affects both crop yield and produce quality. Disease identification is based on visible signs of a pathogen once the plant has already been infected; therefore, techniques that allow objective diagnosis of the disease are currently needed. In this study, the potential of hyperspectral imaging (HSI) technology to assess the presence of powdery mildew in grapevine bunches was evaluated. Thirty Carignan Noir grape bunches, 15 healthy and 15 infected, were analyzed using a lab-scale HSI system (900–1700 nm spectral range). Image processing was performed to extract spectral and spatial image features and then, classification models by means of Partial Least Squares Discriminant Analysis (PLS-DA) were carried out for healthy and infected pixels distinction within grape bunches. The best discrimination was achieved for the PLS-DA model with smoothing (SM), Standard Normal Variate (SNV) and mean centering (MC) pre-processing combination, reaching an accuracy of 85.33% in the cross-validation model and a satisfactory classification and spatial location of either healthy or infected pixels in the external validation. The obtained results suggested that HSI technology combined with chemometrics could be used for the detection of powdery mildew in black grapevine bunches.