Morán Juez, José Fernando

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https://academica-e.unavarra.es/handle/2454/49610

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Morán Juez

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José Fernando

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Ciencias

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IMAB. Research Institute for Multidisciplinary Applied Biology

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0000-0001-6621-6961

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88624

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3087

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Author: Gopalasubramaniama, Sabarinathan K. × Subject: Fluorescence quenching ×

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    PublicationOpen Access
    Soybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1
    (Simplex Academic Publishers, 2013) Gopalasubramaniama, Sabarinathan K.; Kondapallib, Kalyan C.; Millán-Pacheco, César; Pastor, Nina; Stemmler, Timothy L.; Morán Juez, José Fernando; Arredondo-Peter, Raúl; Ciencias del Medio Natural; Natura Ingurunearen Zientziak; Gobierno de Navarra / Nafarroako Gobernua
    Ferrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+ is reduced to Hb2+ by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb13+, however the use of ITC and IEF techniques revealed that this interaction is weak. In silico modeling showed that predicted FLbR2 and native Hb13+ interact at the FAD-binding domain of FLbR2 and the CD-loop and helix F of Hb13+.