Cabodevilla de Andrés, Oihana

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https://academica-e.unavarra.es/handle/2454/48773

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Cabodevilla de Andrés

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Oihana

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Producción Agraria

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8690

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    PublicationOpen Access
    Occlusion body pathogenicity, virulence and productivity traits vary with transmission strategy in a nucleopolyhedrovirus
    (Elservier, 2011-10-25) Cabodevilla de Andrés, Oihana; Ibáñez Elosua, Itxaso; Simón de Goñi, Oihane; Murillo Pérez, Rosa; Caballero Murillo, Primitivo; Williams, Trevor; Producción Agraria; Nekazaritza Ekoizpena; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    The prevalence of sublethal infections of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) was quantified in natural populations of S. exigua in Almería, Spain, during 2006 and 2007. Of 1045 adults collected, 167 (16.1%) proved positive for viral polyhedrin gene transcripts by RT-PCR. The prevalence of covert infection varied significantly according to sex and sample date. Of 1660 progeny of field-collected insects, lethal disease was observed in 10¿33% of offspring of transcript-positive females and 9¿49% of offspring of transcript-negative females. Isolates associated with vertically transmitted infections were characterized by restriction endonuclease analysis using BglII or EcoRV and compared with isolates originating from greenhouse soil-substrate believed to be horizontally transmitted. Insects from a sublethally infected Almerian colony were between 2.3-fold and 4.6-fold more susceptible to infection than healthy insects from a Swiss colony, depending on isolate. Horizontally transmitted isolates were significantly more pathogenic than vertically transmitted isolates in insects from both colonies. Mean speed of kill in second instars (Swiss colony) varied between isolates by >20 h, whereas mean occlusion body (OB) production in fourth instars (Swiss colony) varied by 3.8-fold among isolates. Intriguingly, all three horizontally transmitted isolates were very similar in speed of kill and OB production, whereas all three vertically transmitted isolates differed significantly from one another in both variables, and also differed significantly from the group of horizontally transmitted isolates in speed of kill (one isolate) or both variables (two isolates). We conclude that key pathogenicity and virulence traits of SeMNPV isolates vary according to their principal transmission strategy.
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    PublicationOpen Access
    Intra- and intergenerational persistence of an insect nucleopolyhedrovirus: adverse effects of sublethal disease on host development, reproduction, and susceptibility to superinfection
    (American Society for Microbiology, 2011) Cabodevilla de Andrés, Oihana; Villar Zabal, Eduardo; Virto Garayoa, Cristina; Murillo Pérez, Rosa; Williams, Trevor; Caballero Murillo, Primitivo; Nekazaritza Ekoizpena; Producción Agraria; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Sublethal infections by Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) are common in field populations of the beet armyworm (S. exigua, Hübner) in the Almerian horticultural region of Spain. Inoculation of second, third, and fourth instars with occlusion bodies (OBs) of an isolate (VT-SeAl1) associated with vertically transmitted infections resulted in 15 to 100% of sublethal infection in adult survivors, as determined by reverse transcription-PCR (RT-PCR) detection of viral DNA polymerase transcripts, and quantitative PCR (qPCR) targeted at the DNA polymerase gene. The prevalence of adult sublethal infection was positively related to the inoculum OB concentration consumed during the larval stage. Sublethal infections persisted in OBtreated insects for at least five generations. Viral transcripts were more frequently detected in adult insects than in third instars. qPCR analysis indicated a consistently higher prevalence of sublethal infection than RT-PCR. Sublethal infection was associated with significant reductions in pupal weight, adult emergence, fecundity, and fertility (egg hatch) and significant increases in larval development time and duration of the preoviposition period. Insects taken from a persistently infected experimental population were significantly more susceptible to the OB inoculum than control insects that originated from the same virus-free colony as the persistently infected insects. We conclude that OB treatment results in rapid establishment of sublethal infections that persist between generations and which incur costs in the development and reproductive capacity of the host insect.