Insausti Serrano, Ana María
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Insausti Serrano
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Ana María
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Ciencias de la Salud
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Publication Open Access Quantitative measurements in the human hippocampus and related areas: correspondence between ex-vivo MRI and histological preparations(Public Library of Science, 2015) Delgado González, José Carlos; Mansilla Legorburo, Francisco; Florensa Vila, José; Insausti Serrano, Ana María; Viñuela, Antonio; Tuñón Álvarez, Teresa; Cruz, Marcos; Mohedano Moriano, Alicia; Insausti, Ricardo; Artacho Pérula, Emilio; Ciencias de la Salud; Osasun ZientziakThe decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.Publication Open Access Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies(Frontiers Media, 2023) Insausti, Ricardo; Insausti Serrano, Ana María; Muñoz López, Mónica; Medina Lorenzo, Isidro; Arroyo-Jiménez, María del Mar; Marcos Rabal, Pilar; Rosa-Prieto, Carlos de la; Delgado González, José Carlos; Montón Etxeberria, Javier; Cebada Sánchez, Sandra; Raspeño-García, Juan Francisco; Iñiguez de Onzoño, María Mercedes; Molina Romero, Francisco Javier; Benavides-Piccione, Ruth; Tapia-González, Silvia; Wisse, Laura E. M.; Ravikumar, Sadhana; Wolk, David A.; DeFelipe, Javier; Yushkevich, Paul; Artacho Pérula, Emilio; Ciencias de la Salud; Osasun ZientziakWe present a method for human brain fixation based on simultaneous perfusion of 4% paraformaldehyde through carotids after a flush with saline. The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. The aim of our method is to develop a vascular fixation protocol for the human brain, by adapting protocols that are commonly used in experimental animal studies. We show that a variety of histological procedures can be carried out (cyto- and myeloarchitectonics, histochemistry, immunohistochemistry, intracellular cell injection, and electron microscopy). In addition, ex vivo, ex situ high-resolution MRI (9.4T) can be obtained in the same specimens. This procedure resulted in similar morphological features to those obtained by intravascular perfusion in experimental animals, provided that the postmortem interval was under 10 h for several of the techniques used and under 4 h in the case of intracellular injections and electron microscopy. The use of intravascular fixation of the brain inside the skull provides a fixed whole human brain, perfectly fitted to the skull, with negligible deformation compared to conventional techniques. Given this characteristic of ex vivo, in situ fixation, this procedure can probably be considered the most suitable one available for ex vivo MRI scans of the brain. We describe the compatibility of the method proposed for intravascular fixation of the human brain and fixation of the donor’s body for anatomical purposes. Thus, body donor programs can provide human brain tissue, while the remainder of the body can also be fixed for anatomical studies. Therefore, this method of human brain fixation through the carotid system optimizes the procurement of human brain tissue, allowing a greater understanding of human neurological diseases, while benefiting anatomy departments by making the remainder of the body available for teaching purposes.