(Public Library of Science, 2015) Delgado González, José Carlos; Mansilla Legorburo, Francisco; Florensa Vila, José; Insausti Serrano, Ana María; Viñuela, Antonio; Tuñón Álvarez, Teresa; Cruz, Marcos; Mohedano Moriano, Alicia; Insausti, Ricardo; Artacho Pérula, Emilio; Ciencias de la Salud; Osasun Zientziak
The decrease of volume estimates in different structures of the medial temporal lobe related
to memory correlate with the decline of cognitive functions in neurodegenerative diseases.
This study presents data on the association between MRI quantitative parameters of medial
temporal lobe structures and their quantitative estimate in microscopic examination. Twelve
control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was
sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl
stain was used to establish anatomical boundaries between structures in the medial temporal
lobe. The study included morphometrical and stereological estimates of the amygdaloid
complex, hippocampus, and temporal horn of the lateral ventricle, as well as different
regions of grey and white matter in the temporal lobe. Data showed a close association
between morphometric MRI images values and those based on the histological determination
of boundaries. Only values in perimeter and circularity of the piamater were different.
This correspondence is also revealed by the stereological study, although irregular compartments
resulted in a lesser agreement. Neither age (< 65 yr and > 65yr) nor hemisphere
had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative
information gathered by histological examination, and these data could be used as structural
MRI biomarker in neurodegenerative diseases.
