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Echeverz SarasĂșa, Maite

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https://academica-e.unavarra.es/handle/2454/48933

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Echeverz SarasĂșa

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Maite

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Ciencias de la Salud

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0000-0002-4153-4549

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810062

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2012 - 20172
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Has files: true × Subject: Salmonella × Subject: Biofilm ×

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    PublicationOpen Access
    Salmonella biofilm development depends on the phosphorylation status of RcsB
    (American Society for Microbiology, 2012) Latasa Osta, Cristina; GarcĂ­a MartĂ­nez, Begoña; Echeverz SarasĂșa, Maite; Toledo Arana, Alejandro; Valle Turrillas, Jaione; Campoy SĂĄnchez, Susana; GarcĂ­a del Portillo, Francisco; Solano Goñi, Cristina; Lasa Uzcudun, ĂĂ±igo; IdAB. Instituto de AgrobiotecnologĂ­a / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua: IIM13329.RI1
    The Rcs phosphorelay pathway is a complex signaling pathway involved in the regulation of many cell surface structures in enteric bacteria. In response to environmental stimuli, the sensor histidine kinase (RcsC) autophosphorylates and then transfers the phosphate through intermediary steps to the response regulator (RcsB), which, once phosphorylated, regulates gene expression. Here, we show that Salmonella biofilm development depends on the phosphorylation status of RcsB. Thus, unphosphorylated RcsB, hitherto assumed to be inactive, is essential to activate the expression of the biofilm matrix compounds. The prevention of RcsB phosphorylation either by the disruption of the phosphorelay at the RcsC or RcsD level or by the production of a nonphosphorylatable RcsB allele induces biofilm development. On the contrary, the phosphorylation of RcsB by the constitutive activation of the Rcs pathway inhibits biofilm development, an effect that can be counteracted by the introduction of a nonphosphorylatable RcsB allele. The inhibition of biofilm development by phosphorylated RcsB is due to the repression of CsgD expression, through a mechanism dependent on the accumulation of the small noncoding RNA RprA. Our results indicate that unphosphorylated RcsB plays an active role for integrating environmental signals and, more broadly, that RcsB phosphorylation acts as a key switch between planktonic and sessile life-styles in Salmonella enterica serovar Typhimurium.
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    PublicationOpen Access
    Insights into c-di-GMP signaling and the PGA exopolysaccharide biological functions using Salmonella as a model organism
    (2017) Echeverz SarasĂșa, Maite; Lasa Uzcudun, ĂĂ±igo; Solano Goñi, Cristina; ProducciĂłn Agraria; Nekazaritza Ekoizpena
    Salmonella es un patĂłgeno alimentario de gran relevancia clĂ­nica capaz de adherirse a superficies y formar comunidades bacterianas embebidas en una matriz que ellas mismas producen denominadas biofilms. Esta matriz confiere a las bacterias protecciĂłn frente a agentes externos, aumentando su tolerancia frente a condiciones ambientales adversas, agentes antimicrobianos o el sistema inmune del hospedador. Existe una vĂ­a de señalizaciĂłn, mediada por el dinucleĂłtido cĂ­clico, c-di- GMP, que controla en muchas especies bacterianas la sĂ­ntesis de diversos componentes de la matriz del biofilm, de manera que concentraciones elevadas de este nucleĂłtido activan la producciĂłn de la matriz y por lo tanto del biofilm. La formaciĂłn de biofilms en explotaciones agropecuarias y lugares donde se procesan alimentos es una fuente potencial de contaminaciĂłn y de transmisiĂłn de este patĂłgeno. Diversas medidas de higiene y seguimiento han sido implementadas por las autoridades para el control de esta bacteria; sin embargo alrededor de 93 millones de personas en todo el mundo sufren salmonelosis cada año. Por ello, la bĂșsqueda de medidas alternativas de control, basadas en la vacunaciĂłn animal, asĂ­ como el estudio de los mecanismos de patogenicidad y formaciĂłn de biofilm de Salmonella han sido el objeto de este trabajo.