Ugarte Martínez, María Dolores

Profile Picture

Email Address

person.page.identifierURI

https://academica-e.unavarra.es/handle/2454/50101

Birth Date

Job Title

Last Name

Ugarte Martínez

First Name

María Dolores

person.page.departamento

Estadística, Informática y Matemáticas

person.page.instituteName

InaMat2. Instituto de Investigación en Materiales Avanzados y Matemáticas

ORCID

0000-0002-3505-8400

person.page.observainves

88413

person.page.upna

387

Name

Filters

20211
Reset filters

Settings

Author: Ballekom, Shaf van × Subject: Embryogenic cell lines ×

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    PublicationOpen Access
    Hybrid pine (Pinus attenuata × Pinus radiata) somatic embryogenesis: what do you prefer, mother or nurse?
    (MDPI, 2021) Montalbán, Itziar A.; Castander Olarieta, Ander; Hargreaves, Cathy L.; Gough, Keiko; Reeves, Cathie B.; Ballekom, Shaf van; Goicoa Mangado, Tomás; Ugarte Martínez, María Dolores; Moncaleán, Paloma; Estatistika, Informatika eta Matematika; Institute for Advanced Materials and Mathematics - INAMAT2; Estadística, Informática y Matemáticas; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Development of hybrid pines of Pinus radiata D. Don for commercial forestry presents an opportunity to diversify the current resource of plant material. Climate change and different land uses pose challenges, making alternative species necessary to guarantee wood and non-wood products in the future. Pinus radiata var. cedrosensis × Pinus attenuata hybrid possesses different attributes, such as tolerance to drought conditions, better growth and resistance to snow damage at higher altitudes, and more importantly, different wood quality characteristics. Embryogenic cell lines were successfully initiated reciprocal hybrids using as initial explants megagametophytes, excised zygotic embryos and excised zygotic embryos plus nurse culture. However, the questions raised were: does the initiation environment affect the conversion to somatic plantlets months later? Does the mother tree or the cross have an effect on the conversion to somatic plantlets? In the present work we analysed the maturation rate, number of somatic embryos, germination rate, and the ex-vitro growth in cell lines derived from different initiation treatments, mother tree species, and crosses. Differences were not observed for in vitro parameters such as maturation and germination. However, significant differences were observed due to the mother tree species in relation with the ex-vitro growth rates observed, being higher those in which P. radiata acted as a mother. Moreover, embryogenic cell lines from these hybrids were stored at −80◦C and regenerated after one and five years.