Alfonso Ruiz, Leopoldo

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Alfonso Ruiz

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Leopoldo

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Agronomía, Biotecnología y Alimentación

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IS-FOOD. Research Institute on Innovation & Sustainable Development in Food Chain

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  • PublicationOpen Access
    Molecular traceability of beef from synthetic Mexican bovine breeds
    (FUNPEC-RP, 2011) Rodríguez Ramírez, R.; Arana Navarro, Ana; Alfonso Ruiz, Leopoldo; González Córdova, A. F.; Torrescano, G.; Guerrero Legarreta, I.; Vallejo Córdoba, B.; Producción Agraria; Nekazaritza Ekoizpena
    Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality brands. Molecular meat traceability would allow verification of conventional methods used for beef tracing in synthetic Mexican bovine breeds. We evaluated a set of 11 microsatellites for their ability to identify animals belonging to these synthetic breeds, Brangus and Charolais/Brahman (78 animals). Seven microsatellite markers allowed sample discrimination with a match probability, defined as the probability of finding two individuals sharing by chance the same genotypic profile, of 10-8. The practical application of the marker set was evaluated by testing eight samples from carcasses and pieces of meat at the slaughterhouse and at the point of sale. The DNA profiles of the two samples obtained at these two different points in the productioncommercialization chain always proved that they came from the same animal.