Tryptophan levels as a marker of auxins and nitric oxide signaling

dc.contributor.authorLópez Gómez, Pedro
dc.contributor.authorSmith, Edward N.
dc.contributor.authorBota, Pedro
dc.contributor.authorCornejo Ibergallartu, Alfonso
dc.contributor.authorUrra Rodríguez, Marina
dc.contributor.authorBuezo Bravo, Javier
dc.contributor.authorMorán Juez, José Fernando
dc.contributor.departmentZientziakeu
dc.contributor.departmentInstitute for Advanced Materials and Mathematics - INAMAT2en
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.contributor.departmentCienciases_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2022-05-24T10:30:19Z
dc.date.available2022-05-24T10:30:19Z
dc.date.issued2022
dc.date.updated2022-05-24T10:28:36Z
dc.description.abstractThe aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass- identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling.en
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Science and Innovation (AGL2017-52396-P, co-financed by the European Regional Development Fund, FEDER) and a grant from the Public University of Navarre (Res 309/2022) to J.F.M. M.U. is a recipient of a pre-doctoral fellowship from the Government of Navarre, Spain. J.B. and P.L. have received pre-doctoral fellow- ships from the Public University of Navarre, Spain. J.B. was supported by the Romanian Ministry of Education and research, grant CNCS-UEFISCDI, project number PN-III-P4-ID-PCE-2020-2696, within PNCDI III.en
dc.format.extent18 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.citationLópez-Gómez, P.; Smith, E.N.; Bota, P.; Cornejo, A.; Urra, M.; Buezo, J.; Moran, J.F.. (2022). Tryptophan Levels as a Marker of Auxins and Nitric Oxide Signaling. Plants. 11,10 1304-1304 .en
dc.identifier.doi10.3390/plants1110130
dc.identifier.issn2223-7747
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/43020
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofPlants, 2022, 11 (10),1304-1304en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-52396-P/
dc.relation.publisherversionhttps://doi.org/10.3390/plants1110130
dc.rights© 2022 by the authors. Creative Commons Attribution 4.0en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectTryptophanen
dc.subjectIndole-3-acetaldoximeen
dc.subjectIAOxen
dc.subjectNitric oxideen
dc.subjectNOen
dc.subjectStressen
dc.subjectPrincipal-component analysisen
dc.titleTryptophan levels as a marker of auxins and nitric oxide signalingen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication2e02a0e9-6882-4ace-9d18-07f7043e276e
relation.isAuthorOfPublicationeabef884-715a-460f-b2a1-e62568297b4a
relation.isAuthorOfPublication36b6f084-56a0-4d76-8989-8812a1ed5e80
relation.isAuthorOfPublicationcad13109-2660-4ec4-80c8-e4bcc0685382
relation.isAuthorOfPublication.latestForDiscovery2e02a0e9-6882-4ace-9d18-07f7043e276e

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