Metschnikowia pulcherrima as an efficient biocontrol agent of Botrytis cinerea infection in apples: unraveling protection mechanisms through yeast proteomics

Fernández San Millán, Alicia; Fernández Irigoyen, Joaquín; Santamaría Martínez, Enrique; Larraya Reta, Luis María; Farrán Blanch, Inmaculada; Veramendi Charola, Jon

Publication:
Metschnikowia pulcherrima as an efficient biocontrol agent of Botrytis cinerea infection in apples: unraveling protection mechanisms through yeast proteomics

dc.contributor.author	Fernández San Millán, Alicia
dc.contributor.author	Fernández Irigoyen, Joaquín
dc.contributor.author	Santamaría Martínez, Enrique
dc.contributor.author	Larraya Reta, Luis María
dc.contributor.author	Farrán Blanch, Inmaculada
dc.contributor.author	Veramendi Charola, Jon
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.contributor.department	Institute for Multidisciplinary Research in Applied Biology - IMAB	en
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2023-09-18T16:25:15Z
dc.date.available	2023-09-18T16:25:15Z
dc.date.issued	2023
dc.date.updated	2023-09-18T15:53:24Z
dc.description.abstract	The results obtained in this study show that the Mp-30 strain of Metschnikowia pulcherrima is able to completely prevent Botrytis cinerea infection in apples, which is a major postharvest disease of fruits throughout the world. We have observed that although Mp-30 is able to rapidly colonize wounds, sequestrate iron and secrete antifungal compounds, other unknown mechanisms that occur in the early phase of the yeast-fungal interaction must be implicated in the biocontrol response. The main objective of this study was to identify the pathways involved in the mechanism of action of Mp-30 against B. cinerea in apples. Therefore, differentially accumulated yeast proteins in the presence/absence of B. cinerea on wounded apples were studied to elucidate Mp-30 biocontrol mechanisms and regulation at the protein level. A comparative proteomic analysis showed that 114 yeast proteins were increased and 61 were decreased. The Mp-30 antagonistic response mainly showed the increase of (1) gene expression and protein translation related proteins, (2) trafficking and vesicle-mediated transport related proteins, (3) pyruvate metabolism and mitochondrial proteins related to energy and amino acid production, (4) fatty acid synthesis, and (5) cell envelope related proteins. On the other hand, redox homeostasis, and amino acid and carbon metabolism were downregulated. Since there is no yeast growth enhancement associated with the presence of B. cinerea, such regulation mechanisms may be related to the reprogramming of metabolism, synthesis of new compounds and reorganization of yeast cell structure. Indeed, the results show that several pathways cooperate in restructuring the plasma membrane and cell wall composition, highlighting their major role in the antagonistic interactions for apple protection against gray mold proliferation. These results are of great interest since they provide a clear insight into the yeast mechanisms involved in B. cinerea inactivation during the first hours of contact in the wounded fruit. They shed light on the unknown yeast molecular biocontrol mechanisms.	en
dc.description.sponsorship	This work was financed by the Departamento de Desarrollo Economico y Empresarial from the Government of Navarrre (Spain): grants 0011-1365-2021-000079 and 0011-1411-2019-000009. Open Access funding provided by Universidad Pública de Navarra.	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Fernandez-San Millan, A., Fernandez-Irigoyen, J., Santamaria, E., Larraya, L., Farran, I., Veramendi, J. (2023) Metschnikowia pulcherrima as an efficient biocontrol agent of Botrytis cinerea infection in apples: unraveling protection mechanisms through yeast proteomics. Biological Control, 183, 1-19. https://doi.org/10.1016/j.biocontrol.2023.105266.	en
dc.identifier.doi	10.1016/j.biocontrol.2023.105266
dc.identifier.issn	1049-9644
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/46353
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Biological Control 183 (2023) 105266	en
dc.relation.projectID	info:eu-repo/grantAgreement/Gobierno de Navarra//0011-1365-2021-000079	en
dc.relation.projectID	info:eu-repo/grantAgreement/Gobierno de Navarra//0011-1411-2019-000009	en
dc.relation.publisherversion	https://doi.org/10.1016/j.biocontrol.2023.105266
dc.rights	© 2023 The Author(s). This is an open access article under the CC BY license.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Biocontrol	en
dc.subject	Botrytis	en
dc.subject	Yeast	en
dc.subject	Metschnikowia	en
dc.subject	Proteomic	en
dc.subject	Apple	en
dc.title	Metschnikowia pulcherrima as an efficient biocontrol agent of Botrytis cinerea infection in apples: unraveling protection mechanisms through yeast proteomics	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
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