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Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis

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dc.contributor.authorColomé, Núria
dc.contributor.authorAbian, Joaquín
dc.contributor.authorAloria, Kerman
dc.contributor.authorArizmendi, Jesús M.
dc.contributor.authorBarceló-Batllori, Silvia
dc.contributor.authorBraga-Lagache, Sophie
dc.contributor.authorBurlet-Schiltz, Odile
dc.contributor.authorCarrascal, Montse
dc.contributor.authorCasal, Ignacio J.
dc.contributor.authorChicano-Gálvez, Eduard
dc.contributor.authorChiva, Cristina
dc.contributor.authorClemente, Luis F.
dc.contributor.authorElortza, Félix
dc.contributor.authorEstanyol, Josep M.
dc.contributor.authorFernández Irigoyen, Joaquín
dc.contributor.authorFernández-Puente, Patricia
dc.contributor.authorFidalgo, María J.
dc.contributor.authorFroment, Carine
dc.contributor.authorFuentes, Manuel
dc.contributor.authorFuentes-Almagro, Carlos
dc.contributor.authorGay, Marina
dc.contributor.authorHainard, Alexandre
dc.contributor.authorHeller, Manfred
dc.contributor.authorHernández, María Luisa
dc.contributor.authorIbarrola, Nieves
dc.contributor.authorIloro, Ibon
dc.contributor.authorKieselbach, Thomas
dc.contributor.authorLario, Antonio
dc.contributor.authorLocard-Paulet, Marie
dc.contributor.authorMarina-Ramírez, Anabel
dc.contributor.authorMartín, Luna
dc.contributor.authorMorato-López, Esperanza
dc.contributor.authorMuñoz, Javier
dc.contributor.authorNavajas, Rosana
dc.contributor.authorOdena, Antonia M.
dc.contributor.authorOdriozola, Leticia
dc.contributor.authorOliveira, Eliandre de
dc.contributor.authorParadela, Alberto
dc.contributor.authorPasquarello, Carla
dc.contributor.authorRios, Vivian de los
dc.contributor.authorRuiz-Romero, Cristina
dc.contributor.authorSabidó, Eduard
dc.contributor.authorSánchez del Pino, Manuel
dc.contributor.authorSancho, Jaime
dc.contributor.authorSantamaría Martínez, Enrique
dc.contributor.authorSchaeffer-Reiss, Christine
dc.contributor.authorSchneider, Justine
dc.contributor.authorTorre, Carolina de la
dc.contributor.authorValero, Luz M.
dc.contributor.authorVilaseca, Marta
dc.contributor.authorWu, Shuai
dc.contributor.authorWu, Linfeng
dc.contributor.authorXiménez de Embún, Pilar
dc.contributor.authorCanals, Francesc
dc.contributor.authorCorrales, Fernando J.
dc.contributor.authorProteoRed-ISCIII
dc.contributor.authorEuPA
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.date.accessioned2022-04-12T06:27:05Z
dc.date.available2022-04-12T06:27:05Z
dc.date.issued2022
dc.description.abstractGlobal analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.en
dc.description.sponsorshipProteoRed, PRB3 is supported by grant PT17/0019/0001 , of the PE I+D+i 2013-2016 , funded by ISCIII and ERDF.
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1016/j.jprot.2021.104409
dc.identifier.issn1874-3919
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/42711
dc.language.isoengen
dc.publisherElsevieren
dc.relation.ispartofJournal of Proteomics, 251, 2022en
dc.relation.publisherversionhttps://doi.org/10.1016/j.jprot.2021.104409
dc.rights© 2021 The Authors. Creative Commons Attribution 4.0 Internationalen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPhosphoproteomeen
dc.subjectMass-spectrometryen
dc.subjectProteomicsen
dc.subjectExperimenten
dc.titleMulti-laboratory experiment PME11 for the standardization of phosphoproteome analysisen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
relation.isAuthorOfPublication86d1b76e-4790-40b1-a3ec-72331c5c6199
relation.isAuthorOfPublicationabacfd17-2b93-4d99-bae2-52053d57401e
relation.isAuthorOfPublication.latestForDiscovery86d1b76e-4790-40b1-a3ec-72331c5c6199

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