QTL mapping of pathogenicity in Heterobasidion annosum sensu lato

Date

2006

Authors

Olson, Ake
Lind, Mårten
Stenlid, Jan

Director

Publisher

Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Acceso abierto / Sarbide irekia
Contribución a congreso / Biltzarrerako ekarpena
Versión publicada / Argitaratu den bertsioa

Project identifier

Abstract

Heterobasidion annosum (Fr.) Bref. sensu lato, casual agent of annosum root rot in conifers, is the economical most devastating forest pathogen in the northern hemisphere. A genetic linkage map of H. annosum, was constructed from a compatible mating between isolates from the North American S and P intersterility groups. The linkage analysis of 358 AFLP markers in 102 progeny isolates generated 19 linkage groups containing 6 or more markers that covered 1468 cM. Two distinct methods were used to analyse the segregation of pathogenicity. 1 year old pine seedlings grown in the greenhouse were infected with H. annosum progeny isolates. Pathogenicity was measured as mean necrosis lengths caused in 10 pine plants. One QTL for pathogenicity was found on linkage group 15 with a LOD peak of 2.95, spanning a 31.2 cM large area. This QTL explained 14.3% of the variation. Another QTL were found in a small linkage group containing 5 markers and spanning 36.8 cM, with a LOD peak of 4.40 at marker paacts02. 19.1% of the variation could be explained by this QTL. The heritability of pathogenicity on pine was estimated to be 0.21 in this study. The disease increase rate values from an in vitro test were used as another estimate of pathogenicity and used for the QTL-analysis. From the in vitro pathogenicity test, a large area of 26.5 cM on linkage group 11 between the markers acgcs5 and paacgp08 contains a high QTL probability of LOD 3.09. This QTL explained 16.4% of the total variation in this experiment. The heritability of pathogenicity on pine was estimated to be 0.088 in this study. Successfully localization of the two intersterility genes (S and P) on the map were carried out through mating of the progeny isolates with three tester strains carrying known intersterility genotypes.

Description

Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.

Keywords

Heterobasidion annosum, QTL, Pathogenicity

Department

Faculty/School

Degree

Doctorate program

item.page.cita

item.page.rights

© Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright.

Los documentos de Academica-e están protegidos por derechos de autor con todos los derechos reservados, a no ser que se indique lo contrario.