Study of two acidic proteinases, probably involved in the dimorphism and pathogenicity of Ustilago maydis, basidiomycete of the corn smut disease
| dc.contributor.author | Noriega Reyes, M.Y. | |
| dc.contributor.author | Miramón Martínez, P. | |
| dc.contributor.author | Mercado Flores, Y. | |
| dc.contributor.author | Ramírez Zavala, B. | |
| dc.contributor.author | Hernández Rodríguez, C. | |
| dc.contributor.author | Villa Tanaca, L. | |
| dc.date.accessioned | 2018-02-15T10:32:22Z | |
| dc.date.available | 2018-02-15T10:32:22Z | |
| dc.date.issued | 2006 | |
| dc.description | Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005. | es_ES |
| dc.description.abstract | Ustilago maydis is a dimorphic phythopathogenic fungus and the causal agent of the corn smut disease. In this work, the purification and biochemical characterization of the acid proteinases pumAe (extracellular) and pumAi (intracellular) of U. maydis were performed. Also, identity of the gene that encodes for pumAi (PRAum) was explored in the genome of the fungus. The proteases were purified and biochemically characterized. The molecular masses of pumAe and pumAi were 72 and 35.3 kDa respectively. The optimal pH of activity of proteinases was 4.0. The pumAe Km value was of 3.5 μM and a Vmax of 11430 μmol h-1 mg-1 when Suc-R-P-F-H-L-L-V-Y-MCA was used as substrate. The protease pumAi was inhibited by pepstatine A. Yeast-tomycelium transition was inhibited by Pepstatine A in the culture medium. The hypothetical gene that encodes for protease pumAi (PRAum gene) was located in the U. maydis genome project and was amplified by PCR and cloned into TOPO-TA 2.1 plasmid and pNMT-1, a Schizosaccharomyces pombe expression vector. In the U. maydis genome one copy of the gene by Southern blot analyses was detected. In brief, the expression of this gene (PRAum), performed by RT-PCR assays, was regulated by the source of nitrogen. The heterologous expression experiments in S. pombe allowed a fast purification and confirmed that pumAi enzymatic activity was encoded by PRAum gene. | en |
| dc.format.extent | 1 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.isbn | 84-9769-107-5 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/27263 | |
| dc.language.iso | eng | en |
| dc.publisher | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.relation.ispartof | Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006. | es |
| dc.rights | © Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright. | es_ES |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Ustilago maydis | en |
| dc.subject | pumAe | en |
| dc.subject | pumAi | en |
| dc.title | Study of two acidic proteinases, probably involved in the dimorphism and pathogenicity of Ustilago maydis, basidiomycete of the corn smut disease | en |
| dc.type | info:eu-repo/semantics/conferenceObject | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication |