Molecular analysis of two acidic proteinases pumAe and pumAi and aminopeptidase pumAPE from Ustilago maydis: enzymes purification and differential expression

dc.contributor.authorNoriega Reyes, M.Y.
dc.contributor.authorMiramón Martínez, P.
dc.contributor.authorMercado Flores, Y.
dc.contributor.authorRamírez Zavala, B.
dc.contributor.authorHernández Rodríguez, C.
dc.contributor.authorVilla Tanaca, L.
dc.date.accessioned2018-02-14T09:31:32Z
dc.date.available2018-02-14T09:31:32Z
dc.date.issued2006
dc.descriptionComunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.es_ES
dc.description.abstractProteolytic system of Ustilago maydis was recently partially described (Mercado-Flores et al., 2003). Two acidic proteinases pumAe (extracellular) and pumAi (intracellular) and aminopeptidase pumAPE were detected and purified from the haploid phase of U. maydis. Purification consisted of ammonium sulphate fractionation and different chromatographic steps. Molecular masses were estimated: 58 kDa for pumAPE, 72 kDa for pumAe and 35.3 kDa for pumAi. Enzymatic activity was optimal at pH 7.0 and 35 ºC for pumAPE and 4.0 for the two proteinases. pumAPE was inhibited by EDTA-Na2, 1,10-phenanthroline, bestantin, PMSF and several divalent cations, while proteinase pumAi was inhibited by pepstatine A, also finding that yeast-to-mycelium transition was inhibited by Pepstatine A in the culture medium. Primers were designed in order to amplify the gene APEum encoding pumAPE and PRAum gene encoding pumAi, and they were used as probes in a Southern blot. One copy of each gene was detected by genome in several strains. Differential expression of APEum was assessed under different physiological conditions, detecting high expression levels on media supplemented with corn infusion, proline, peptone and ammonium sulphate. PRAum is expressed when cells are exposed to corn infusion and ammonium sulphate.en
dc.description.sponsorshipC. Hernández-Rodríguez and L. Villa-Tanaca received COFAA, and EDI (IPN) supports. This work was supported by grant CGPI 20050005, IPN, Mexico. L. Villa- Tanaca was hired by “Programa Institucional de Contratación de Personal Académico de Excelencia para el Reforzamiento del Posgrado, IPN”.en
dc.format.extent13 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.isbn84-9769-107-5
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/27224
dc.language.isoengen
dc.publisherUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.relation.ispartofAntonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.es
dc.rights© Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright.es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectUstilago maydisen
dc.subjectMolecular analysisen
dc.titleMolecular analysis of two acidic proteinases pumAe and pumAi and aminopeptidase pumAPE from Ustilago maydis: enzymes purification and differential expressionen
dc.typeinfo:eu-repo/semantics/conferenceObject
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication

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