Expression of GFP and DsRed in the homobasidiomycete Schizophyllum commune
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Expression of GFP and DsRed was studied in the homobasidiomycete Schizophyllum commune. CGFP or sGFP fused to the SC3 promoter resulted in similar steady state mRNA levels. These levels were considerably lower than that of SC3. Despite the low mRNA levels, both GFP variants resulted in fluorescent hyphae. The sGFP expressing strains showed stronger fluorescence than the CGFP expressers. When CGFP was fused to the N-terminal or C-terminal part of the mature SC3 protein, no fluorescence was observed. However, accumulation of GFP
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