Biochemistry of volatile compounds synthesis in Agaricus bisporus

dc.contributor.authorCombet, E.
dc.contributor.authorHenderson, J.
dc.contributor.authorEastwood, D.C.
dc.contributor.authorGriffiths, G.
dc.contributor.authorBurton, K.S.
dc.date.accessioned2018-02-14T09:31:34Z
dc.date.available2018-02-14T09:31:34Z
dc.date.issued2006
dc.descriptionResumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.es_ES
dc.description.abstractAgaricus bisporus unique flavour is due to the release of a set of eight-carbon volatile compounds, which biosynthetic pathway has not been elucidated yet, despite of the numerous implications of those volatile compounds. Beside their influence on crop quality, they are also important for insect perception and play a part in triggering the switch from vegetative to reproductive growth in mushrooms. 8-carbon volatiles are derived from the oxygenation and the cleavage of the polyunsaturated fatty acid linoleic acid. This reaction has similarities to the plant system, but also major differences. Examination of the enzymic mechanisms and the fatty acid chemistry suggested that the enzyme involved in the oxygenation step could be a lipoxygenase (as found in plants) or a heme-dioxygenase, similar to the recently isolated linoleate diol synthase from Gaeumannomyces graminis. In order to characterise the biochemical pathway leading to eight-carbon volatile production, we investigated fatty acid and lipids distribution in Agaricus bisporus, as well as hydroperoxide and volatile compounds levels. In parallel, we searched for candidate genes susceptible to encode the enzyme responsible for this novel oxidation route in fungi. The combination of analytical methods, such as GC-MS, with a molecular approach based on degenerate PCR and library screening provided us with a broad range of results. These results establish the relation between fatty acids and volatile compounds and enabled us to gain a better understanding of mushroom volatiles biosynthesis and lipid metabolism.en
dc.format.extent1 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.isbn84-9769-107-5
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/27234
dc.language.isoengen
dc.publisherUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.relation.ispartofAntonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.es
dc.rights© Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright.es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectAgaricus bisporusen
dc.subjectVolatile compoundsen
dc.titleBiochemistry of volatile compounds synthesis in Agaricus bisporusen
dc.typeinfo:eu-repo/semantics/conferenceObject
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication

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