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RinA controls phage-mediated packaging and transfer of virulence genes in gram-positive bacteria

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dc.contributor.authorFerrer, María Desamparados
dc.contributor.authorQuiles Puchalt, Nuria
dc.contributor.authorHarwich, Michael D.
dc.contributor.authorTormo Más, María Ángeles
dc.contributor.authorCampoy Sánchez, Susana
dc.contributor.authorBarbé, Jordi
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorNovick, Richard P.
dc.contributor.authorChristie, Gail E.
dc.contributor.authorPenadés, José R.
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2014-06-10T06:51:33Z
dc.date.available2014-06-10T06:51:33Z
dc.date.issued2011
dc.description.abstractPhage-mediated transfer of microbial genetic elements plays a crucial role in bacterial life style and evolution. In this study, we identify the RinA family of phage-encoded proteins as activators required for transcription of the late operon in a large group of temperate staphylococcal phages. RinA binds to a tightly regulated promoter region, situated upstream of the terS gene, that controls expression of the morphogenetic and lysis modules of the phage, activating their transcription. As expected, rinA deletion eliminated formation of functional phage particles and significantly decreased the transfer of phage and pathogenicity island encoded virulence factors. A genetic analysis of the late promoter region showed that a fragment of 272 bp contains both the promoter and the region necessary for activation by RinA. In addition, we demonstrated that RinA is the only phage-encoded protein required for the activation of this promoter region. This region was shown to be divergent among different phages. Consequently, phages with divergent promoter regions carried allelic variants of the RinA protein, which specifically recognize its own promoter sequence. Finally, most Gram-postive bacteria carry bacteriophages encoding RinA homologue proteins. Characterization of several of these proteins demonstrated that control by RinA of the phage-mediated packaging and transfer of virulence factor is a conserved mechanism regulating horizontal gene transfer.en
dc.description.sponsorshipFunding: Consolider-Ingenio CSD2009-00006, BIO2005-08399- C02-02, BIO2008-05284-C02-02 and BIO2008-00642-E/C from the Ministerio de Ciencia e Innovación (MICINN), and grants from the Cardenal Herrera-CEU University (PRCEU-UCH39/10 and Copernicus-Banco Santander program), from the Conselleria de Agricultura, Pesca i Alimentació (CAPiA) and from the Generalitat Valenciana (ACOMP07/258) to J.R.P., grants [BFU2008-01078] from the MICINN and [2009SGR1106] from the Generalitat de Catalunya to J.B., NIH grants [R21 AI067654 and R56 AI081837] and a grant-in-aid from the A.D. Williams Trust and the Baruch Foundation Trust to G.E.C., a VCU Graduate School Thesis and Dissertation Award to M.D.H., and NIH grant [R01AI022159-23A2] to R.P.N. Funding for open access charge: Consolider-Ingenio CSD2009-00006, BIO2005-08399-C02-02, BIO2008-05284-C02-02 and BIO2008-00642-E/C from the Ministerio de Ciencia e Innovación (MICINN), Spain.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1093/nar/gkr158
dc.identifier.issn0305-1048 (print)
dc.identifier.issn1362-4962 (electronic)
dc.identifier.other561
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/10792
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofNucleic acids research, 2011, 39(14). Págs. 5866-5878en
dc.relation.publisherversionhttps://dx.doi.org/10.1093/nar/gkr158
dc.rights© The Author(s) 2011. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5/
dc.subjectBacteriophage T4 late promoteren
dc.subjectPanton Valentine leukocidinen
dc.subjectPathogenicity island SAPI1en
dc.subjectViral late transcriptionen
dc.subjectColien
dc.subjectRNA-Polymeraseen
dc.subjectStaphylococcus aureusen
dc.subjectSequence analysisen
dc.subjectBinding proteinsen
dc.subjectAlpha subuniten
dc.subjectMom promoteren
dc.titleRinA controls phage-mediated packaging and transfer of virulence genes in gram-positive bacteriaen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoveryc654d104-1ae2-41cf-9215-4b4bed3e5ea6

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