Investigations into the fungal-fungal interaction between Verticillium fungicola and Agaricus bisporus
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Plant and animal pathogens section
The cultivated button mushroom, Agaricus bisporus, is amenable to number
of pathogenic threats including bacteria, viruses, mites, insects and fungi.
Currently, the most significant threat to the commercial mushroom industry
is the mycoparasite, Verticillium fungicola. Infection by V. fungicola can
drastically reduce the yield and value of mushroom crops. T ...
[++]
Plant and animal pathogens section
The cultivated button mushroom, Agaricus bisporus, is amenable to number
of pathogenic threats including bacteria, viruses, mites, insects and fungi.
Currently, the most significant threat to the commercial mushroom industry
is the mycoparasite, Verticillium fungicola. Infection by V. fungicola can
drastically reduce the yield and value of mushroom crops. The severity of this
disease is dependent on the developmental stage of A. bisporus at the time of
infection and is manifested in three types of symptoms: spotty cap, stipe
blowout and dry bubble. An aim of our research has been to develop molecular
tools for V. fungicola that will allow us to study the interaction between
this pathogen and A. bisporus. These tools have included transformation
methods, marker gene techniques as well as gene-knockout technologies.
This has involved the use of Agrobacterium and T-DNA to introduce disruption
constructs into V. fungicola as part of a molecular investigation into
this fungal-fungal interaction. We have developed an efficient transformation
system for V. fungicola that we have now adapted to give high levels of
targeted mutagenesis. This technique has successfully generated targeted
mutants of a β-1-6 glucanase homologue from Trichoderma harzianum and a
Mitogen Activated Protein Kinase homologue (PMK1) from Magnaporthe
grisea identified using degenerate PCR primers. We have also developed TDNA
tagging technology in a mycology context for random mutagenesis in
V. fungicola. [--]
Materias
Verticillum fungicola,
Agaricus bisporus
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.