A novel binary mixture of Helicoverpa armigera single nucleopolyhedrovirus genotypic variants has improved insecticidal characteristics for control of cotton bollworms
Fecha
2015Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.00339-15
Resumen
The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated
with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for control of the cotton bollworm.
Two genotypic variants, HearSP1A and HearSP1B, were cloned in vitro from the most pathogenic wild-type isolate of the Iberian
Peninsula, HearS ...
[++]
The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated
with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for control of the cotton bollworm.
Two genotypic variants, HearSP1A and HearSP1B, were cloned in vitro from the most pathogenic wild-type isolate of the Iberian
Peninsula, HearSNPV-SP1 (HearSP1-wt). Similarly, six genotypic variants (HearLB1 to -6) were obtained by endpoint dilution
from larvae collected from cotton crops in southern Spain that died from virus disease during laboratory rearing. Variants differed
significantly in their insecticidal properties, pathogenicity, speed of kill, and occlusion body (OB) production (OBs/larva).
HearSP1B was ~3-fold more pathogenic than HearSP1-wt and the other variants. HearLB1, HearLB2, HeaLB5, and HearLB6
were the fastest-killing variants. Moreover, although highly virulent, HearLB1, HearLB4, and HearLB5 produced more OBs/
larva than did the other variants. The co-occluded HearSP1B:LB6 mixture at a 1:1 proportion was 1.7- to 2.8-fold more pathogenic
than any single variant and other mixtures tested and also killed larvae as fast as the most virulent genotypes. Serial passage
resulted in modified proportions of the component variants of the HearSP1B:LB6 co-occluded mixture, suggesting that
transmissibility could be further improved by this process. We conclude that the improved insecticidal phenotype of the
HearSP1B:LB6 co-occluded mixture underlines the utility of the genotypic variant dissection and reassociation approach for the
development of effective virus-based insecticides. [--]
Materias
Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV),
Cotton bollworm,
Virus-based insecticides
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, 81:3984 –3993
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study received financial support from the Gobierno de Navarra
(project IIQ14065:RI1). M.A. received a predoctoral fellowship from
CSIC.