Potential of the Bacillus thuringiensis toxin reservoir for the control of Lobesia botrana (Lepidoptera: tortricidae), a major pest of grape plants
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.01511-06
Resumen
The potential of Bacillus thuringiensis Cry proteins to control the grape pest Lobesia botrana was explored by
testing first-instar larvae with Cry proteins belonging to the Cry1, Cry2, and Cry9 groups selected for their
documented activities against Lepidoptera. Cry9Ca, a toxin from B. thuringiensis, was the protein most toxic to
L. botrana larvae, followed in decreasing order by Cry2Ab, Cry1Ab, ...
[++]
The potential of Bacillus thuringiensis Cry proteins to control the grape pest Lobesia botrana was explored by
testing first-instar larvae with Cry proteins belonging to the Cry1, Cry2, and Cry9 groups selected for their
documented activities against Lepidoptera. Cry9Ca, a toxin from B. thuringiensis, was the protein most toxic to
L. botrana larvae, followed in decreasing order by Cry2Ab, Cry1Ab, Cry2Aa, and Cry1Ia7, with 50% lethal
concentration values of 0.09, 0.1, 1.4, 3.2, and 8.5 μg/ml of diet, respectively. In contrast, Cry1Fa and Cry1JA
were not active at the assayed concentration (100 μg/ml). In vitro binding and competition experiments showed
that none of the toxins tested (Cry1Ia, Cry2Aa, Cry2Ab, and Cry9C) shared binding sites with Cry1Ab. We
conclude that either Cry1Ia or Cry9C could be used in combination with Cry1Ab to control this pest, either as
the active components of B. thuringiensis sprays or expressed together in transgenic plants. [--]
Materias
Bacillus thuringiensis,
Lobesia botrana,
Cry proteins
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Jan. 2007, Vol. 73, No. 1, p. 337–340
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This study was supported by the Spanish Ministry of Science and Technology
(AGL2000-0840-C03 and AGL2003-09282-CO3). I.R.D.E., A.E.,
and B.E. received support from the Government of Navarre, fellowship
FP2000-5497, and the Ramón y Cajal program, respectively.