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dc.creatorGopalasubramaniama, Sabarinathan K.es_ES
dc.creatorKondapallib, Kalyan C.es_ES
dc.creatorMillán-Pacheco, Césares_ES
dc.creatorPastor, Ninaes_ES
dc.creatorStemmler, Timothy L.es_ES
dc.creatorMorán Juez, José Fernandoes_ES
dc.creatorArredondo-Peter, Raúles_ES
dc.date.accessioned2019-06-07T11:00:07Z
dc.date.available2019-06-07T11:00:07Z
dc.date.issued2013
dc.identifier.urihttps://hdl.handle.net/2454/33266
dc.description.abstractFerrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+ is reduced to Hb2+ by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb13+, however the use of ITC and IEF techniques revealed that this interaction is weak. In silico modeling showed that predicted FLbR2 and native Hb13+ interact at the FAD-binding domain of FLbR2 and the CD-loop and helix F of Hb13+.en
dc.description.sponsorshipThis work was funded by CoNaCyT (project no. 42873Q), México (to R. A.-P.), Dept. of Education, Government of Navarre (no. 999–2006), Spain (to J. F. M.) and NIDDKD (DK068139), USA (to T. L. S.).en
dc.format.extent13 p.
dc.language.isoengen
dc.publisherSimplex Academic Publishersen
dc.relation.ispartofScienceJet 2013, 2: 33en
dc.subjectFluorescence quenchingen
dc.subjectHemoglobinen
dc.subjectIsothermal calorimetryen
dc.subjectNon-symbioticen
dc.subjectOryza sativaen
dc.subjectProtein-protein interactionen
dc.titleSoybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1en
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Ciencias del Medio Naturales_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes


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